Evolutionary genomics of KPC-2-producing Serratia marcescens and characterization of a novel blaKPC-2-harboring plasmid
文献类型：期刊论文
| 作者 | Zheng, Yali2; Jing, Ying3; Zhang, Yan2; Zhang, Jin4; Ye, Jianbo4; Chen, Huimin4; Jin, Yuting4; He, Jiayu5; Zhang, Yu1; Luo, Xinhua4 |
| 刊名 | BMC MICROBIOLOGY
 |
| 出版日期 | 2025-12-26 |
| 卷号 | 26期号:1页码:14 |
| 关键词 | |
| ISSN号 | 1471-2180 |
| DOI | 10.1186/s12866-025-04617-w |
| 通讯作者 | Luo, Xinhua(luoxiangnanchang84@163.com) |
| 英文摘要 | BackgroundSerratia marcescens is a common opportunistic pathogen in hospitals. Its carbapenem-resistant strains, especially those carrying the blaKPC-2 gene, pose a serious challenge to clinical infection treatment.MethodsIn this study, we identified 14 strains that were positive for blaKPC-2 from tertiary hospitals in Zhejiang Province, China, through phenotypic tests and sequencing. We also incorporated data from the GenBank public database, resulting in a total of 2005 Serratia strains included for bioinformatic analysis.ResultsA total of 121 strains (6.03%) were found to carry blaKPC-2. These positive isolates predominantly originated from China (76.86%) and the United States (14.88%), with the vast majority (94.21%) identified as clinical isolates. The period from 2018 to 2019 was relatively active for global data collection, particularly for samples from the Yangtze River Delta region in China. Phylogenetic analysis classified the 121 strains carrying blaKPC-2 into six evolutionary clusters (Cluster I-VI), with most strains grouped in Cluster V and Cluster VI. Cluster VI, largely consisting of ST595, was predominantly composed of Chinese isolates. In contrast, Cluster V displayed coexistence of diverse sequence types and geographic origins. Resistance gene profiling identified 52 antimicrobial resistance genes. Besides blaKPC-2, three strains were also positive for blaNDM-1. Additionally, a novel plasmid lineage was identified and designated as pK17-KPC/pK1030-KPC (approximately 87 kb). Its replication initiator gene, repA1, showed low homology (<= 93.25%) to known plasmids. This plasmid has a complex structure and carries a blaKPC-2 resistance module mediated by unit transposon Delta Tn6296.ConclusionsThis study systematically describes the global transmission patterns and molecular genetic features of Serratia marcescens strains carrying blaKPC-2. It reveals the structural characteristics of a novel plasmid harboring blaKPC-2 and provides key scientific evidence for clinical prevention and control of antimicrobial resistance. |
| WOS关键词 | RESISTANCE ; ELEMENT |
| 资助项目 | Natural Science Foundation of Zhejiang Province[LTGY23H190003] ; Medical and Health Science and Technology Project of Zhejiang Province[2024KY543] ; Medical and Health Science and Technology Project of Zhejiang Province[2025KY461] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001679392900001 |
| 出版者 | BMC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/322740]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Luo, Xinhua |
| 作者单位 | 1.Soochow Univ, Sch Life Sci, Suzhou Med Coll, Suzhou, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 3.Capital Med Univ, Beijing Childrens Hosp, Dept Clin Lab Ctr, Natl Ctr Childrens Hlth, Beijing, Peoples R China 4.Taizhou Univ, Taizhou Univ Affiliated Municipal Hosp, Taizhou Municipal Hosp, Dept Clin Lab Med,Sch Med, Taizhou, Peoples R China 5.Taizhou Jiaojiang Dist Maternal & Child Hlth Care, Taizhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Yali,Jing, Ying,Zhang, Yan,et al. Evolutionary genomics of KPC-2-producing Serratia marcescens and characterization of a novel blaKPC-2-harboring plasmid[J]. BMC MICROBIOLOGY,2025,26(1):14. |
| APA | Zheng, Yali.,Jing, Ying.,Zhang, Yan.,Zhang, Jin.,Ye, Jianbo.,...&Luo, Xinhua.(2025).Evolutionary genomics of KPC-2-producing Serratia marcescens and characterization of a novel blaKPC-2-harboring plasmid.BMC MICROBIOLOGY,26(1),14. |
| MLA | Zheng, Yali,et al."Evolutionary genomics of KPC-2-producing Serratia marcescens and characterization of a novel blaKPC-2-harboring plasmid".BMC MICROBIOLOGY 26.1(2025):14. |
入库方式： OAI收割
来源：上海药物研究所
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