Isolation and whole-genome sequencing of antibiotic-resistant bacteria revealed the reservoir for indigenous antibiotic resistance genes in the deepest ocean sediment of the Challenger Deep
文献类型：期刊论文
| 作者 | Ding, Wenmian3; Wang, Yuepeng3; Ma, Yuewei3; Chen, Ping2; Yang, Ji3; Song, Zhiping3; Wang, Yuguo3; Zhang, Wenju3; Li, Xuan4; Huang, Yanyan1 |
| 刊名 | MARINE POLLUTION BULLETIN
 |
| 出版日期 | 2026-05-01 |
| 卷号 | 226页码:15 |
| 关键词 | Antibiotic-resistant bacteria Antibiotic resistance genes The Challenger Deep |
| ISSN号 | 0025-326X |
| DOI | 10.1016/j.marpolbul.2025.119206 |
| 通讯作者 | Li, Xuan(lixuan@sippe.ac.cn) ; Huang, Yanyan(huangyy@fudan.edu.cn) ; Nan, Peng(nanpeng@fudan.edu.cn) |
| 英文摘要 | Understanding the occurrence of antibiotic-resistant bacteria (ARB) and associated antibiotic resistance genes (ARGs) in remote marine environments is crucial for accessing treats of ARG pollution on a border ecological scale. While most studies focused on anthropogenically disturbed settings, the Challenger Deep, as the deepest ocean habitat, offers a unique opportunity to investigate minimally disturbed resistomes. We revived 123 bacterial isolates from the Challenger Deep sediment, assessed their antibiotic susceptibility, and identified their taxonomy via 16S rRNA gene sequencing. Among them, 96 strains (78.0%) were resistant to at least one antibiotic, with high prevalence observed in Halomonas, Idiomarina, Flagellimonas, and Microbacterium. Resistance was most common to ampicillin (73.2%), followed by sulfadiazine (30.1%) and nalidixic acid (4.9%). Untargeted metabolomics identified 359 metabolites in the sediment sample, including 6-aminopenicillanic acid, suggesting local microbial antibiotic production and selective pressure of resistance. Anthropogenic contaminants like nalidixic acid were also detected. Whole-genome sequencing of eight representative ARB strains revealed 77 copies of 26 ARG subtypes, predominantly associated with multidrug resistance and efflux pump mechanisms. Notably, no mobile genetic elements were linked to ARGs, indicating limited horizontal gene transfer. Phylogenetic analyses showed host species specificity of ARGs, independent of geography or environmental context, supporting vertical inheritance from ancestral lineages. This study offers the first culture-based evidence of ARB and ARGs in the Challenger Deep, suggesting that resistance may represent an adaptive trait to extreme conditions and underscoring its ancient, intrinsic origin. Our findings provide critical implications for understanding the revolution and dissemination of resistance in deep-sea environments. |
| WOS关键词 | BETA-LACTAMASES ; SP NOV. ; ENVIRONMENT ; IDIOMARINA ; SOFTWARE ; ESTUARY ; FATE ; ARGS |
| 资助项目 | National Natural Science Foundation of China[92451303] ; National Natural Science Foundation of China[32200093] ; National Key Research and Development Program of China[2018YFC0310600] |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001679827300001 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/322758]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, Xuan; Huang, Yanyan; Nan, Peng |
| 作者单位 | 1.Fudan Univ, Inst Preservat & Conservat Chinese Ancient Books, Shanghai 200433, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 3.Fudan Univ, State Key Lab Wetland Conservat & Restorat, Inst Ecochongming,Minist Educ Key Lab Biodivers Sc, Sch Life Sci,Natl Observat & Res Stn Wetland Ecosy, Shanghai 200438, Peoples R China 4.Chinese Acad Sci, Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, Key Lab Synthet Biol, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Wenmian,Wang, Yuepeng,Ma, Yuewei,et al. Isolation and whole-genome sequencing of antibiotic-resistant bacteria revealed the reservoir for indigenous antibiotic resistance genes in the deepest ocean sediment of the Challenger Deep[J]. MARINE POLLUTION BULLETIN,2026,226:15. |
| APA | Ding, Wenmian.,Wang, Yuepeng.,Ma, Yuewei.,Chen, Ping.,Yang, Ji.,...&Nan, Peng.(2026).Isolation and whole-genome sequencing of antibiotic-resistant bacteria revealed the reservoir for indigenous antibiotic resistance genes in the deepest ocean sediment of the Challenger Deep.MARINE POLLUTION BULLETIN,226,15. |
| MLA | Ding, Wenmian,et al."Isolation and whole-genome sequencing of antibiotic-resistant bacteria revealed the reservoir for indigenous antibiotic resistance genes in the deepest ocean sediment of the Challenger Deep".MARINE POLLUTION BULLETIN 226(2026):15. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。