Single-molecule Investigation of Heparan Sulfate-metal Ion Interactions Using Aerolysin Nanopore
文献类型：期刊论文
| 作者 | Zhang, Pengling3; Gao, Fan4; Chen, Jiale1; Zou, Aihua3; Tang, Juan5; Ma, Hui6; Jiang, Cuiling2; Wan, Yongjing2; Xia, Bingqing1; Li, Tiehai1 |
| 刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| 出版日期 | 2026-02-10 |
| 卷号 | 47期号:2页码:6 |
| 关键词 | Aerolysin Single-molecule sensing Nanopore Heparan sulfate Metal ion |
| ISSN号 | 0251-0790 |
| DOI | 10.7503/cjcu20250405 |
| 通讯作者 | Zou, Aihua(aihuazou@shnu.edu.cn) |
| 英文摘要 | Heparin(HP) and heparan sulfate(HS) are highly anionic glycosaminoglycans that play essential roles in diverse biological processes through the metal ion-mediated interactions with proteins. However, direct characterization of HS-metal ion interactions at the single-molecule level in solution remains challenging. Nanopore electrochemistry is a label-free and single-molecule technique that enables direct analysis of individual molecular interactions. In this study, a T232K/K238Q Aerolysin nanopore featuring an enhanced electrostatic repelling barrier was utilized to probe the interactions between HS and different metal ions. By systematically varying the electrolyte cations(Na+, K+, and Ca2+), we have found that the metal ions significantly regulate HS translocation behavior by modulating its conformation, charge screening, and HS-nanopore interactions. Notably, in addition to Ca2+, which exhibits strong binding affinity to HS, the monovalent cations Na+ and K+ with similar physicochemical properties and weaker binding also induce distinct single-molecule signal signatures. Our results demonstrate that the nanopore-based single-molecule analysis holds strong potential to resolve the fine structural features of HS, enabling the characterization of sulfation site distributions, repeat-unit lengths, and related sequence features, and thereby providing a new avenue for high-resolution analysis of complex glycans. |
| WOS关键词 | BINDING ; OLIGONUCLEOTIDE ; GLYCANS |
| 资助项目 | Shanghai Municipal Science and Technology Major Project, China ; National Natural Science Foundation of China[32250019] ; National Natural Science Foundation of China[22474055] ; Special Fund for the Basic Scientific Research of Central Universities, China[020514380356] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001682869300006 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/322827]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zou, Aihua |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.East China Univ Sci & Technol, Sch Informat Sci & Engn, Shanghai 200237, Peoples R China 3.Shanghai Normal Univ, Coll Chem & Mat Sci, Shanghai 200234, Peoples R China 4.Nanjing Univ, Mol Sensing & Imaging Ctr, Sch Chem, Nanjing 210023, Peoples R China 5.Jiangxi Normal Univ, Coll Chem & Mat, Nanchang 330022, Peoples R China 6.Zhejiang Sci Tech Univ, Sch Chem & Chem Engn, Hangzhou 310018, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Pengling,Gao, Fan,Chen, Jiale,et al. Single-molecule Investigation of Heparan Sulfate-metal Ion Interactions Using Aerolysin Nanopore[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2026,47(2):6. |
| APA | Zhang, Pengling.,Gao, Fan.,Chen, Jiale.,Zou, Aihua.,Tang, Juan.,...&Long, Yitao.(2026).Single-molecule Investigation of Heparan Sulfate-metal Ion Interactions Using Aerolysin Nanopore.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,47(2),6. |
| MLA | Zhang, Pengling,et al."Single-molecule Investigation of Heparan Sulfate-metal Ion Interactions Using Aerolysin Nanopore".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 47.2(2026):6. |
入库方式： OAI收割
来源：上海药物研究所
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