Chemical Proteomic Profiling of Lysine Benzoylation
文献类型：期刊论文
| 作者 | Song, Xiaohan3; Lu, Yuhan2; Peng, Panpan1; Chen, Binjing3; Huang, He1,2,3,4 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2026-02-04 |
| 页码 | 9 |
| 关键词 | lysine benzoylation substrates chemical proteomic nucleophosmin |
| ISSN号 | 1535-3893 |
| DOI | 10.1021/acs.jproteome.5c00792 |
| 通讯作者 | Huang, He(hhuang@simm.ac.cn) |
| 英文摘要 | Benzoylation (Kbz) is a physiologically relevant post-translational modification derived from the food additive sodium benzoate. While Kbz has been implicated in unique cellular regulatory processes, its substrate landscape and functional consequences remain poorly characterized. Conventional antibody-based enrichment methods for Kbz detection suffer from affinity bias and limited specificity. Here, we developed AyBz3, a bioorthogonal chemical probe enabling the unbiased mapping of Kbz across the proteome. Implementation of AyBz3 in HepG2 cells revealed 688 unique Kbz sites, significantly expanding the known benzoylome. Functional analysis revealed that Kbz-modified proteins are enriched in pathways related to protein translation and cell adhesion. Notably, we demonstrated that Kbz modification of nucleophosmin 1 (NPM1) impairs its molecular chaperone function toward p53, resulting in accelerated p53 degradation. Together, this study establishes AyBz3 as a powerful probe for unbiased benzoylome profiling and provides new insights into the regulatory roles of Kbz in cellular processes. |
| WOS关键词 | METABOLIC-REGULATION ; GENE-EXPRESSION ; NUCLEOPHOSMIN ; REPORTERS |
| 资助项目 | Chinese Academy of Sciences[XDB1060000] ; Taishan Scholar Project of Shandong Province[tstp20240845] ; Science and Technology Commission of Shanghai Municipality[NA] ; Department of Science and Technology of Shandong Province[SYS202205] ; Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences[NA] ; Hangzhou Municipal Government[NA] ; Natural Science Foundation of Shanghai Municipality[23ZR1474600] ; Natural Science Foundation of Shandong Province[ZR2024MB112] ; Key Technology Research and Development Program of Shandong Province[2024CXPT028] ; National Natural Science Foundation of China[22277125] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001683972500001 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/322842]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Huang, He |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Chem Biol, Shanghai 201203, Peoples R China 2.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China 3.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Key Lab Glyco Drug Res Zhejiang Prov, Hangzhou 310024, Peoples R China 4.Bohai Rim Adv Res Inst Drug Discovery, Shandong Lab Yantai Drug Discovery, Yantai 264117, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Xiaohan,Lu, Yuhan,Peng, Panpan,et al. Chemical Proteomic Profiling of Lysine Benzoylation[J]. JOURNAL OF PROTEOME RESEARCH,2026:9. |
| APA | Song, Xiaohan,Lu, Yuhan,Peng, Panpan,Chen, Binjing,&Huang, He.(2026).Chemical Proteomic Profiling of Lysine Benzoylation.JOURNAL OF PROTEOME RESEARCH,9. |
| MLA | Song, Xiaohan,et al."Chemical Proteomic Profiling of Lysine Benzoylation".JOURNAL OF PROTEOME RESEARCH (2026):9. |
入库方式： OAI收割
来源：上海药物研究所
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