Developmental dynamics of bronchioalveolar stem cells revealed by dual-reporter lineage tracing
文献类型：期刊论文
| 作者 | Li, Yuanshu2,6; Liu, Jiaqi3; Chi, Shuilian3; Lv, Rongrong1,2,5; Wang, Haowen1,2,5; Jiang, Li2; Xie, Yulong2,4; Wang, Chaoqun1,2,3,5,6 |
| 刊名 | RESPIRATORY RESEARCH
 |
| 出版日期 | 2026-01-13 |
| 卷号 | 27期号:1页码:15 |
| 关键词 | BASCs Lung development Dual recombinase system (Cre-Dre) Lineage tracing Stromal niche |
| DOI | 10.1186/s12931-026-03498-5 |
| 英文摘要 | Bronchioalveolar stem cells (BASCs), located at the bronchioalveolar duct junction (BADJ), contribute to adult lung regeneration after injury, yet their role during postnatal lung morphogenesis remains poorly characterized. In this study, we showed that BASCs first emerged at birth and persisted throughout the postnatal saccular stage with stable localization and abundance. To trace their fate, we generated a SftpcDreER knock-in mouse and combined it with Scgb1a1CreER and dual-reporter H11LSL - ZsG_RSR-tdT mice to establish a dual recombinase-mediated lineage tracing system (BASC-Tracer). Using this system, we observed that BASCs expanded postnatally and migrated into both alveolar and airway compartments. In the alveoli, they lost SCGB1A1 expression and differentiated into AT2 and AT1 cells to drive alveolar maturation, whereas in the airways, they lost SFTPC expression and gave rise to club cells. Furthermore, we employed a hyperoxia-induced bronchopulmonary dysplasia model to assess the injury response of BASCs during lung development. We found that hyperoxic exposure markedly suppresses BASC regenerative capacity. Finally, spatial transcriptomic analysis revealed that BASCs reside within a distinct BADJ-associated niche, characterized by a unique repertoire of signaling ligands that distinguishes this niche from those of AT2 and club cells. Together, these findings delineate the dynamic behavior of BASCs during lung development and provide spatial characterization of their niche. |
| WOS关键词 | LUNG ; DISEASE ; REPAIR |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDB0830000] ; Major Project of Guangzhou National Laboratory[GZNL2023A01003] ; National Key Research and Development Program of China[2024YFA1107703] ; Zhongshan Science and Technology Bureau[CXTD2023009] |
| WOS研究方向 | Respiratory System |
| 语种 | 英语 |
| WOS记录号 | WOS:001686263100005 |
| 出版者 | BMC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/322845]  |
| 专题 | 国家级研究中心_原创新药研究全国重点实验室 |
| 通讯作者 | Xie, Yulong; Wang, Chaoqun |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528400, Peoples R China 3.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China 4.Zhongshan City Peoples Hosp, Dept Nasopharyngeal Head & Neck Tumor Radiotherapy, 2 Sunwen East Rd, Zhongshan 528400, Guangdong, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China 6.Guangzhou Univ Chinese Med, Sch Chinese Mat Med, Guangzhou 510006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yuanshu,Liu, Jiaqi,Chi, Shuilian,et al. Developmental dynamics of bronchioalveolar stem cells revealed by dual-reporter lineage tracing[J]. RESPIRATORY RESEARCH,2026,27(1):15. |
| APA | Li, Yuanshu.,Liu, Jiaqi.,Chi, Shuilian.,Lv, Rongrong.,Wang, Haowen.,...&Wang, Chaoqun.(2026).Developmental dynamics of bronchioalveolar stem cells revealed by dual-reporter lineage tracing.RESPIRATORY RESEARCH,27(1),15. |
| MLA | Li, Yuanshu,et al."Developmental dynamics of bronchioalveolar stem cells revealed by dual-reporter lineage tracing".RESPIRATORY RESEARCH 27.1(2026):15. |
入库方式： OAI收割
来源：上海药物研究所
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