Protein-encapsulated fluorogenic probes for the selective detection of endogenous O-GlcNAcase (OGA)
文献类型：期刊论文
| 作者 | Wu, Yuan-Hao3; Guo, Chen2,3; Ye, Zi-Ru2,3; Hu, Xi-Le3; James, Tony D.1,5; Li, Jia2; He, Xiao-Peng3,4 |
| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2026-02-17 |
| 页码 | 7 |
| ISSN号 | 2041-6520 |
| DOI | 10.1039/d5sc06843f |
| 英文摘要 | Ever-increasing evidence confirms the role of O-GlcNAcase (OGA) in mediating cell growth and development as well as pathology and underscores the importance of developing sensitive and selective chemical tools for the study of OGA biology. Here, based on our previously developed protein-encapsulation strategy, we designed and synthesized a series of fluorogenic probes based on resorufin, and assembled their composites with human serum albumin (HSA) for the selective detection of endogenous OGA activity in live cells. We show that host-guest self-assembly with HSA significantly enhances the OGA sensitivity of the probes in aqueous solution and cells. The structure of the complex of a glycoprobe and HSA was resolved by small-angle X-ray scattering. We demonstrate that the replacement of the acetyl group in GlcNAc with a propionyl group results in selectivity for OGA over hexosaminidases (HEX) that unselectively hydrolyze hexosamines. This allows us to differentiate between two cell lines with different endogenous OGA and HEX expression levels, and selectively detect OGA activity in live cells. |
| WOS关键词 | INHIBITOR ; ALBUMIN |
| 资助项目 | Fundamental Research Funds for the Central Universities[222201717003] ; Science and Technology Commission of Shanghai Municipality[24DX1400200] ; State Key Laboratory of Chemo/Biosensing and Chemometrics[Unassigned] ; Henan Normal University[2020ZD01] ; Hunan University[Unassigned] ; University of Bath[Unassigned] ; National Natural Science Foundation of China[92253306] ; National Natural Science Foundation of China[82130099] ; National Natural Science Foundation of China[22477030] ; Dalian University of Technology[Unassigned] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001692137500001 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/323041]  |
| 专题 | 国家级研究中心_原创新药研究全国重点实验室 |
| 通讯作者 | James, Tony D.; Li, Jia; He, Xiao-Peng |
| 作者单位 | 1.Univ Bath, Dept Chem, Bath BA2 7AY, England 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai 201203, Peoples R China 3.East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, Key Lab Adv Mat Joint & Int Res Lab Precis Chem &, 130 Meilong Rd, Shanghai 200237, Peoples R China 4.Natl Ctr Liver Canc, Eastern Hepatobiliary Surg Hosp, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China 5.Henan Normal Univ, Sch Chem & Chem Engn, Xinxiang 453007, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Yuan-Hao,Guo, Chen,Ye, Zi-Ru,et al. Protein-encapsulated fluorogenic probes for the selective detection of endogenous O-GlcNAcase (OGA)[J]. CHEMICAL SCIENCE,2026:7. |
| APA | Wu, Yuan-Hao.,Guo, Chen.,Ye, Zi-Ru.,Hu, Xi-Le.,James, Tony D..,...&He, Xiao-Peng.(2026).Protein-encapsulated fluorogenic probes for the selective detection of endogenous O-GlcNAcase (OGA).CHEMICAL SCIENCE,7. |
| MLA | Wu, Yuan-Hao,et al."Protein-encapsulated fluorogenic probes for the selective detection of endogenous O-GlcNAcase (OGA)".CHEMICAL SCIENCE (2026):7. |
入库方式： OAI收割
来源：上海药物研究所
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