The allochthonous macroflora of the Middle Jurassic (Bathonian) Arda Formation in Northwest Jordan has previously been described based on morphological features of plant remains, without reference to microstructures (i.e., cuticles). This study examines seven types of cuticle from the same flora, all of which belong to gymnosperms. The identified cuticles include bennettitaleans (Otozamites sp. and Bennettitales spp.), and conifers (scale-leaved conifer and probably conifers with longitudinally arranged stomatal rows). The discovery of cuticles provides the first record of conifers in the Middle Jurassic flora of NW Jordan, filling a gap in the previous knowledge. The macroflora, comprising sphenophytes, ferns, bennettitaleans, and conifers (including scale-leaved conifers), shows strong similarities to other Jurassic floras in the Middle East, particularly resembling that of the Middle Jurassic Imran Formation of Makhtesh Ramon in the Negev (Southern Israel). These additions to the known facts about the floral assemblage by microstructural analyses significantly provide more information to support existing reconstructions of the palaeoenvironmental and palaeoclimatic conditions in NW Jordan during the Middle Jurassic.