The Estrogenic Activity of Isoflavones Extracted from Chickpea Cicer arietinum L Sprouts in Vitro
文献类型：期刊论文
| 作者 | Ma HaiRong; Wei HuaBo; Chen Zhen; Yang Yi; Wang ZhengHua; Madina Habasi ; Cao Xu; Akber Aisa Haji
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| 刊名 | PHYTOTHERAPY RESEARCH
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| 出版日期 | 2013 |
| 卷号 | 27期号:8页码:1237-1242 |
| 关键词 | Cicer arietinum L sprouts isoflavones estrogenic activity |
| ISSN号 | 0951-418X |
| 中文摘要 | Isoflavones have drawn attention due to their potential therapeutic use. Isoflavones are the important chemical components of the seeds and sprouts of chickpea and higher isoflavones in sprouts than in seeds. However, there have been no previous reports of the estrogenic activity of isoflavones extracted from chickpea Cicer arietinum L sprouts (ICS) in vitro. In this study, which incorporated several in vitro bioassays methods, we systematically evaluated the estrogenic properties of ICS. MTT assay showed that ICS at the low concentration ranges (10(-3)-1mg/L) promoted MCF-7 cell growth, while at high concentrations, (>1mg/L) inhibited cell proliferation, indicating ICS worked at a diphasic mechanism. Flow cytometric analysis further calculated the proliferation rate of ICS at low concentration (1mg/L). ER/Luc trans-activation assay and then semi-quantitative RT-PCR analysis indicated that ICS at low concentrations induced ER-mediated luciferase activity in MCF-7 cells and promoted the ER downstream target gene pS2 and PR trans-activation. These effects were inhibited by ICI 182,780, a special antagonist of ER, indicating that an ER-mediating pathway was involved. Alkaline phosphatase (AP) expression in Ishikawa cells showed that ICS at low concentrations stimulated AP expression. Our current study is the first to demonstrate that ICS has significant estrogenic activity in vitro. ICS may be useful as a supplement to hormone replacement therapy and in dietary supplements. Copyright (c) 2012 John Wiley & Sons, Ltd. |
| 学科主题 | Pharmacology & Pharmacy |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China 81102890; CAS/SAFEA International Partnership Program for Creative Research Teams KGCX2-YW-503; Natural Science Foundation of Xinjiang Province, China 2010211A58; China National Science Fund for Distinguished Young Scholars 30925045 |
| 公开日期 | 2013-11-07 |
| 源URL | [http://ir.xjipc.cas.cn/handle/365002/2643]  |
| 专题 | 新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室 新疆理化技术研究所_资源化学研究室 |
| 作者单位 | Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Peoples Hosp Fengdu Cty Chongqing City, Chongqing 408200, Peoples R China;Shihezi Univ, Coll Pharm, Minist Educ, Key Lab Xinjiang Endem Phytomed Resources, Shihezi 832002, Peoples R China;Johns Hopkins Univ, Sch Med, Dept Orthopaed Surg, Baltimore, MD 21205 USA |
| 推荐引用方式 GB/T 7714 | Ma HaiRong,Wei HuaBo,Chen Zhen,et al. The Estrogenic Activity of Isoflavones Extracted from Chickpea Cicer arietinum L Sprouts in Vitro[J]. PHYTOTHERAPY RESEARCH,2013,27(8):1237-1242. |
| APA | Ma HaiRong.,Wei HuaBo.,Chen Zhen.,Yang Yi.,Wang ZhengHua.,...&Akber Aisa Haji.(2013).The Estrogenic Activity of Isoflavones Extracted from Chickpea Cicer arietinum L Sprouts in Vitro.PHYTOTHERAPY RESEARCH,27(8),1237-1242. |
| MLA | Ma HaiRong,et al."The Estrogenic Activity of Isoflavones Extracted from Chickpea Cicer arietinum L Sprouts in Vitro".PHYTOTHERAPY RESEARCH 27.8(2013):1237-1242. |
入库方式： OAI收割
来源：新疆理化技术研究所
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