Improving the Stability of Immobilized Penicillin G Acylase via the Modification of Supports With Ionic Liquids
文献类型：期刊论文
| 作者 | Zhou, Huacong1,2; Yang, Liangrong1; Li, Wei1; Shou, Qinghui1,2; Xu, Peng1,2; Li, Wensong1,2 ; Wang, Fuchun1,2; Yu, Pinhua1,2; Liu, Huizhou1
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| 刊名 | INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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| 出版日期 | 2012-03-28 |
| 卷号 | 51期号:12页码:4582-4590 |
| 关键词 | COATED MAGNETIC NANOPARTICLES CARBON-DIOXIDE METAL-IONS ADSORPTION LIPASE MICROSPHERES CONVERSION CATALYSTS MEMBRANE SOLVENTS |
| ISSN号 | 0888-5885 |
| 通讯作者 | Li, W |
| 英文摘要 | With the aim of improving the stability of penicillin G acylase (PGA), functional ionic liquids (ILs) were used to modify the surface chemistry of the supports on which they were physically adsorbed. Four kinds of ILs-specifically, 1-methyl-3-(triethoxysilylpropyl)-imidazolium salts, with Cl-, BF4-, PF6-, and Tf2N- as the anions (IL-Cl-, BF4-, PF6-, and Tf2N-, respectively)-were used to tune the hydrophilic or hydrophobic properties of the ILs. The synthesized ILs were first immobilized on magnetic silica nanoparticles (Fe3O4/SiO2), and the composite material (Fe3O4/SiO2-IL) was then applied to immobilize PGA via physical adsorption. The amount of protein loading, the specific activity, the immobilization yield, and the stability of immobilized PGA were investigated to evaluate the effects of the Its on the PGA immobilization. The results showed that PGA immobilized on Fe3O4/SiO2-ILs was more stable than that immobilized on Fe3O4/SiO2 with no IL modification. Among the four kinds of ILs tested, the hydrophilic ILs (IL-Cl-, and IL-BF4-) were superior to the hydrophobic ones (IL-PF6-, and IL-Tf2N-) for PGA immobilization. The reusability of the immobilized PGA on Fe3O4/SiO2-IL (BF4-) was evaluated; a high residual activity (70% of the initial activity) was observed after 9 consecutive operation cycles under the experimental conditions. This activity was 1.8 times higher than that of immobilized PGA on naked Fe3O4/SiO2 (40%). |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Chemical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | COATED MAGNETIC NANOPARTICLES ; CARBON-DIOXIDE ; METAL-IONS ; ADSORPTION ; LIPASE ; MICROSPHERES ; CONVERSION ; CATALYSTS ; MEMBRANE ; SOLVENTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301968300014 |
| 公开日期 | 2013-10-22 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3860]  |
| 专题 | 过程工程研究所_湿法冶金清洁生产技术国家工程实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Huacong,Yang, Liangrong,Li, Wei,et al. Improving the Stability of Immobilized Penicillin G Acylase via the Modification of Supports With Ionic Liquids[J]. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,2012,51(12):4582-4590. |
| APA | Zhou, Huacong.,Yang, Liangrong.,Li, Wei.,Shou, Qinghui.,Xu, Peng.,...&Liu, Huizhou.(2012).Improving the Stability of Immobilized Penicillin G Acylase via the Modification of Supports With Ionic Liquids.INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,51(12),4582-4590. |
| MLA | Zhou, Huacong,et al."Improving the Stability of Immobilized Penicillin G Acylase via the Modification of Supports With Ionic Liquids".INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH 51.12(2012):4582-4590. |
入库方式： OAI收割
来源：过程工程研究所
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