Acyl homoserine lactone-based quorum sensing in a methanogenic archaeon
文献类型：期刊论文
| 作者 | Zhang, Guishan1; Zhang, Fan1; Ding, Gang2,3; Li, Jie1; Guo, Xiaopeng1; Zhu, Jinxing1; Zhou, Liguang1; Cai, Shichun1; Liu, Xiaoli1; Luo, Yuanming1 |
| 刊名 | ISME JOURNAL
 |
| 出版日期 | 2012-07-01 |
| 卷号 | 6期号:7页码:1336-1344 |
| 关键词 | carboxylated acyl homoserine lactones filI-encoded AHL synthase methanogenic archaea physiology transition quorum sensing |
| ISSN号 | 1751-7362 |
| 通讯作者 | Dong, XZ |
| 英文摘要 | Acyl homoserine lactone (AHL)-based quorum sensing commonly refers to cell density-dependent regulatory mechanisms found in bacteria. However, beyond bacteria, this cell-to-cell communication mechanism is poorly understood. Here we show that a methanogenic archaeon, Methanosaeta harundinacea 6Ac, encodes an active quorum sensing system that is used to regulate cell assembly and carbon metabolic flux. The methanogen 6Ac showed a cell density-dependent physiology transition, which was related to the AHL present in the spent culture and the filI gene-encoded AHL synthase. Through extensive chemical analyses, a new class of carboxylated AHLs synthesized by FilI protein was identified. These carboxylated AHLs facilitated the transition from a short cell to filamentous growth, with an altered carbon metabolic flux that favoured the conversion of acetate to methane and a reduced yield in cellular biomass. The transcriptomes of the filaments and the short cell forms differed with gene expression profiles consistent with the physiology. In the filaments, genes encoding the initial enzymes in the methanogenesis pathway were upregulated, whereas those for cellular carbon assimilation were downregulated. A luxI-luxR ortholog filI-filR was present in the genome of strain 6Ac. The carboxylated AHLs were also detected in other methanogen cultures and putative filI orthologs were identified in other methanogenic genomes as well. This discovery of AHL-based quorum sensing systems in methanogenic archaea implies that quorum sensing mechanisms are universal among prokaryotes. The ISME Journal (2012) 6, 1336-1344; doi:10.1038/ismej.2011.203; published online 12 January 2012 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Ecology ; Microbiology |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Microbiology |
| 关键词[WOS] | N-ACYLHOMOSERINE LACTONES ; LINEAR ION-TRAP ; LTQ-FTICR-MS ; MASS-SPECTROMETRY ; PSEUDOMONAS-AERUGINOSA ; METHANOSARCINA-MAZEI ; UASB REACTOR ; BACTERIA ; AUTOINDUCER ; ACETATE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000305631100008 |
| 公开日期 | 2013-10-16 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3578]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Microbial Resources, Inst Microbiol, Beijing 100101, Peoples R China 2.Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100730, Peoples R China 3.Peking Union Med Coll, Beijing 100021, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100101, Peoples R China 5.Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA USA |
| 推荐引用方式 GB/T 7714 | Zhang, Guishan,Zhang, Fan,Ding, Gang,et al. Acyl homoserine lactone-based quorum sensing in a methanogenic archaeon[J]. ISME JOURNAL,2012,6(7):1336-1344. |
| APA | Zhang, Guishan.,Zhang, Fan.,Ding, Gang.,Li, Jie.,Guo, Xiaopeng.,...&Dong, Xiuzhu.(2012).Acyl homoserine lactone-based quorum sensing in a methanogenic archaeon.ISME JOURNAL,6(7),1336-1344. |
| MLA | Zhang, Guishan,et al."Acyl homoserine lactone-based quorum sensing in a methanogenic archaeon".ISME JOURNAL 6.7(2012):1336-1344. |
入库方式： OAI收割
来源：过程工程研究所
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