Intensification of beta-poly(l-malic acid) production by Aureobasidium pullulans ipe-1 in the late exponential growth phase
文献类型：期刊论文
| 作者 | Cao, Weifeng1,2; Luo, Jianquan1; Zhao, Juan3; Qiao, Changsheng3; Ding, Luhui4; Qi, Benkun1; Su, Yi1; Wan, Yinhua1 |
| 刊名 | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2012-07-01 |
| 卷号 | 39期号:7页码:1073-1080 |
| 关键词 | beta-Poly(malic acid) Aureobasidium pullulans Cell-recycle culture Culture redox potential |
| ISSN号 | 1367-5435 |
| 通讯作者 | Su, Y |
| 英文摘要 | beta-Poly(malic acid) (PMLA) has attracted industrial interest because this polyester can be used as a prodrug or for drug delivery systems. In PMLA production by Aureobasidium pullulans ipe-1, it was found that PLMA production was associated with cell growth in the early exponential growth phase and dissociated from cell growth in the late exponential growth phase. To enhance PMLA production in the late phase, different fermentation modes and strategies for controlling culture redox potential (CRP) were studied. The results showed that high concentrations of produced PMLA (above 40 g/l) not only inhibited PMLA production, but also was detrimental to cell growth. Moreover, when CRP increased from 57 to 100 mV in the late exponential growth phase, the lack of reducing power in the broth also decreased PMLA productivity. PMLA productivity could be enhanced by repeated-batch culture to maintain cell growth in the exponential growth phase, or by cell-recycle culture with membrane to remove the produced PMLA, or by maintaining CRP below 70 mV no matter which kind of fermentation mode was adopted. Repeated-batch culture afforded a high PMLA concentration (up to 63.2 g/l) with a productivity of 1.15 g l(-1) h(-1). Cell-recycle culture also confirmed that PMLA production by the strain ipe-1 was associated with cell growth. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | WHOLE-CELL BIOCATALYST ; L-MALIC ACID ; ESCHERICHIA-COLI ; SACCHAROMYCES-CEREVISIAE ; PHYSARUM-POLYCEPHALUM ; POLY(BETA-MALIC ACID) ; FERMENTATION ; POLYMERASE ; OXIDATION ; INVITRO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000305522500012 |
| 公开日期 | 2013-10-16 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3579]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Tianjin Univ Sci & Technol, Dept Bioengn, Tianjin 300457, Peoples R China 4.Univ Technol Compiegne, EA TIMR 4297, Biol Engn Dept, F-60205 Compiegne, France |
| 推荐引用方式 GB/T 7714 | Cao, Weifeng,Luo, Jianquan,Zhao, Juan,et al. Intensification of beta-poly(l-malic acid) production by Aureobasidium pullulans ipe-1 in the late exponential growth phase[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2012,39(7):1073-1080. |
| APA | Cao, Weifeng.,Luo, Jianquan.,Zhao, Juan.,Qiao, Changsheng.,Ding, Luhui.,...&Wan, Yinhua.(2012).Intensification of beta-poly(l-malic acid) production by Aureobasidium pullulans ipe-1 in the late exponential growth phase.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,39(7),1073-1080. |
| MLA | Cao, Weifeng,et al."Intensification of beta-poly(l-malic acid) production by Aureobasidium pullulans ipe-1 in the late exponential growth phase".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 39.7(2012):1073-1080. |
入库方式： OAI收割
来源：过程工程研究所
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