Cross-Linked Enzyme Aggregates of Phenylalanine Ammonia Lyase: Novel Biocatalysts for Synthesis of L-Phenylalanine
文献类型：期刊论文
| 作者 | Cui, Jian-Dong1,2,3; Zhang, Si2; Sun, Li-Mei2 |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2012-06-01 |
| 卷号 | 167期号:4页码:835-844 |
| 关键词 | Phenylalanine ammonia lyase Rhodotorula glutinis Cross-linked enzyme aggregates L-phenylalanine Biocatalysis |
| ISSN号 | 0273-2289 |
| 通讯作者 | Cui, JD |
| 英文摘要 | Cross-linked enzyme aggregates of phenylalanine ammonia lyase (PAL-CLEAs) from Rhodotorula glutinis were prepared. The effects of the type of aggregating agent, its concentration, and that of cross-linking agent were studied. PAL-CLEAs production was most effective using ammonium sulfate (40 % saturation), followed by cross-linking for 1 h with 0.2 % (v/v) glutaraldehyde. Moreover, the storage and operational stability of the resulting PAL-CLEAs were also investigated. Compared to the free enzyme, the PAL-CLEAs exhibited the expected increased stability of the enzyme against various deactivating conditions such as pH, temperature, denaturants, and organic solvents and showed higher storage stability than its soluble counterpart. Additionally, the reusability of PAL-CLEAs with respect to the biotransformation of l-phenylalanine was evaluated. PAL-CLEAs could be recycled at least for 12 consecutive batch reactions without dramatic activity loss, which should dramatically increase the commercial potential of PAL for synthesis of l-phenylalanine. To the best of our knowledge, this is the first report of immobilization of PAL as cross-linked enzyme aggregates. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | RECOMBINANT ESCHERICHIA-COLI ; RHODOTORULA-GLUTINIS ; ORGANIC-SOLVENTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000304930200013 |
| 公开日期 | 2013-10-17 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3595]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Key Lab Ind Microbiol, Minist Educ, Tai Da Dev Area, Tianjin 300457, Peoples R China 2.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Res Ctr Fermentat Engn Hebei, Shijiazhuang 050018, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Jian-Dong,Zhang, Si,Sun, Li-Mei. Cross-Linked Enzyme Aggregates of Phenylalanine Ammonia Lyase: Novel Biocatalysts for Synthesis of L-Phenylalanine[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2012,167(4):835-844. |
| APA | Cui, Jian-Dong,Zhang, Si,&Sun, Li-Mei.(2012).Cross-Linked Enzyme Aggregates of Phenylalanine Ammonia Lyase: Novel Biocatalysts for Synthesis of L-Phenylalanine.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,167(4),835-844. |
| MLA | Cui, Jian-Dong,et al."Cross-Linked Enzyme Aggregates of Phenylalanine Ammonia Lyase: Novel Biocatalysts for Synthesis of L-Phenylalanine".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 167.4(2012):835-844. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。