Purification and characterization of a nitroreductase from the soil bacterium Streptomyces mirabilis
文献类型：期刊论文
| 作者 | Yang, Jun1; Xie, Bo2 ; Bai, Jing1; Yang, Qing1
|
| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2012-05-01 |
| 卷号 | 47期号:5页码:720-724 |
| 关键词 | Biotransformation Nitroreductase Nitroaromatic compounds Streptomyces |
| ISSN号 | 1359-5113 |
| 通讯作者 | Yang, Q |
| 英文摘要 | A NADH-dependent nitroreductase from an efficient nitro-reducing soil bacterium, Streptomyces mirabilis DUT001, was isolated and characterized. The enzyme was purified to near homogeneity using ammonium sulfate precipitation, ion exchange chromatography, and gel filtration chromatography. The native enzyme was estimated by gel filtration to have a molecular weight of 68 kDa, and its subunit molecular weight determined by SDS-PAGE was about 34 kDa, which indicated this enzyme was a dimer. Polycyclic nitroaromatic compounds were preferred substrates for this enzyme. The purified enzyme exhibited maximum activity at pH 7.5 and 40 degrees C. The addition of various chemicals such as reducing agents, metal ions, and chelating agents, had effects on enzyme activity. Mg2+, Ca2+, Sr2+, and 1% (w/v) Triton X-100 increased activity. However. Hg2+, Co2+, Ni2+, Cu2+, and SDS reduced activity. The maximum reaction rate (V-max) was 64 mu M min(-1) mg(-1) enzyme and the apparent Michaelis-Menten constants (K-m) for 4-nitro-1,8-naphthalic anhydride and NADH were 276 and 29 mu M, respectively. Menadione, bimethylenebis, sodium benzoate, and antimycin A were inhibitors of the purified nitroreductase with apparent inhibition constants (K(i)s) of 20, 36, 44 and 80 mu M, respectively. (C) 2012 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | NITROAROMATIC COMPOUNDS ; ENTEROBACTER-CLOACAE ; ESCHERICHIA-COLI ; 2,4,6-TRINITROTOLUENE ; BIODEGRADATION ; REDUCTION ; SEQUENCE ; CLONING ; ENZYME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000303622400006 |
| 公开日期 | 2013-10-18 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3819]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Jun,Xie, Bo,Bai, Jing,et al. Purification and characterization of a nitroreductase from the soil bacterium Streptomyces mirabilis[J]. PROCESS BIOCHEMISTRY,2012,47(5):720-724. |
| APA | Yang, Jun,Xie, Bo,Bai, Jing,&Yang, Qing.(2012).Purification and characterization of a nitroreductase from the soil bacterium Streptomyces mirabilis.PROCESS BIOCHEMISTRY,47(5),720-724. |
| MLA | Yang, Jun,et al."Purification and characterization of a nitroreductase from the soil bacterium Streptomyces mirabilis".PROCESS BIOCHEMISTRY 47.5(2012):720-724. |
入库方式： OAI收割
来源：过程工程研究所
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