Optimization of the mated fermentation process for the production of lycopene by Blakeslea trispora NRRL 2895 (+) and NRRL 2896 (-)
文献类型：期刊论文
| 作者 | Wang, Jin-Feng1; Liu, Xiu-Ji1; Liu, Rui-Sang1; Li, Hong-Mei1; Tang, Ya-Jie1,2,3,4 |
| 刊名 | BIOPROCESS AND BIOSYSTEMS ENGINEERING
 |
| 出版日期 | 2012-05-01 |
| 卷号 | 35期号:4页码:553-564 |
| 关键词 | Lycopene The mated fermentation of Blakeslea trispora Process optimization |
| ISSN号 | 1615-7591 |
| 通讯作者 | Tang, YJ |
| 英文摘要 | The mated fermentation process for the production of lycopene by Blakeslea trispora NRRL 2895 (+) and NRRL 2896 (-) was systematically optimized in shake flasks. The ratio of the (+) to (-) strains, the lycopene cyclase inhibitors piperidine and creatinine, the trisporic acid structural analog abscisic acid, the 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) precursor leucine, and the mevalonate kinase enhancer penicillin were all identified as key factors affecting lycopene biosynthesis. With an optimal ratio of 5:1 for the (+) to (-) strains and the addition of 6 g/L creatinine on day 3, the highest lycopene production was 98.1 +/- A 15.5 mg/L. Based on the above result, the addition of 0.1 g/L penicillin on day 4, 150 mu mol/L abscisic acid on day 3 or 0.5 g/L leucine on day 4 enhanced lycopene production to 119.7 +/- A 17.2, 120.6 +/- A 12.3 and 135.2 +/- A 7.0 mg/L, respectively. Finally, an integrated strategy by combining the above key factors was developed, and the highest lycopene production of 156.2 +/- A 15.4 mg/L was obtained, which was enhanced by 134.9% comparing with its production of 66.5 +/- A 3.6 mg/L before the optimization process of this work. The results obtained in this study may be useful for large-scale industrial lycopene production. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | BETA-CAROTENE ; PHYCOMYCES-BLAKESLEEANUS ; BIOSYNTHESIS ; CAROTENOGENESIS ; ACID ; CULTURES ; STRAINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000303053900008 |
| 公开日期 | 2013-10-18 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3828]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Hubei Univ Technol, Minist Educ, Key Lab Fermentat Engn, Wuhan 430068, Peoples R China 2.Chinese Acad Sci, Shanghai Adv Res Inst, Lab Biorefinery Engn, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 4.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jin-Feng,Liu, Xiu-Ji,Liu, Rui-Sang,et al. Optimization of the mated fermentation process for the production of lycopene by Blakeslea trispora NRRL 2895 (+) and NRRL 2896 (-)[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2012,35(4):553-564. |
| APA | Wang, Jin-Feng,Liu, Xiu-Ji,Liu, Rui-Sang,Li, Hong-Mei,&Tang, Ya-Jie.(2012).Optimization of the mated fermentation process for the production of lycopene by Blakeslea trispora NRRL 2895 (+) and NRRL 2896 (-).BIOPROCESS AND BIOSYSTEMS ENGINEERING,35(4),553-564. |
| MLA | Wang, Jin-Feng,et al."Optimization of the mated fermentation process for the production of lycopene by Blakeslea trispora NRRL 2895 (+) and NRRL 2896 (-)".BIOPROCESS AND BIOSYSTEMS ENGINEERING 35.4(2012):553-564. |
入库方式： OAI收割
来源：过程工程研究所
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