Improved Mass Multiplication of Rhodiola crenulata Shoots Using Temporary Immersion Bioreactor with Forced Ventilation
文献类型：期刊论文
| 作者 | Zhao, Yan1; Sun, Wei2; Wang, Ying3; Saxena, Praveen K.4; Liu, Chun-Zhao1 |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2012-03-01 |
| 卷号 | 166期号:6页码:1480-1490 |
| 关键词 | Rhodiola crenulata Temporary immersion bioreactor Forced ventilation Shoot propagation |
| ISSN号 | 0273-2289 |
| 通讯作者 | Liu, CZ |
| 英文摘要 | A temporary immersion bioreactor system was found to be suitable for mass shoot proliferation of Rhodiola crenulata. The shoot multiplication ratio and hyperhydration rate reached 46.8 and 35.4%, respectively, at a temporary immersion cycle of 3-min immersion every 300 min. Forced ventilation was employed in the temporary immersion bioreactor culture in order to decrease the hyperhydration rate, improve shoot quality and enhance the multiplication ratio. The highest multiplication ratio of 55.7 was obtained under a temporary immersion cycle of 3-min immersion every 180 min with the forced ventilation at an air flow rate of 40 l/h, and the hyperhydration rate was reduced to 26.1%. Forced ventilation also improved the subsequent elongation and rooting rate of these proliferated shoots, and the shoot cultures from the temporary immersion bioreactor formed complete plantlets when subcultured onto a rooting medium containing 5 mu mol/l indole-3-acetic acid. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | CELL-CULTURES ; GROWTH ; MICROPROPAGATION ; SYSTEM ; PLANTLETS ; ANATOMY ; POTATO ; WATER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000302770300011 |
| 公开日期 | 2013-10-22 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3852]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Gen Hosp AF, Beijing 100142, Peoples R China 3.Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, S China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China 4.Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada |
| 推荐引用方式 GB/T 7714 | Zhao, Yan,Sun, Wei,Wang, Ying,et al. Improved Mass Multiplication of Rhodiola crenulata Shoots Using Temporary Immersion Bioreactor with Forced Ventilation[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2012,166(6):1480-1490. |
| APA | Zhao, Yan,Sun, Wei,Wang, Ying,Saxena, Praveen K.,&Liu, Chun-Zhao.(2012).Improved Mass Multiplication of Rhodiola crenulata Shoots Using Temporary Immersion Bioreactor with Forced Ventilation.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,166(6),1480-1490. |
| MLA | Zhao, Yan,et al."Improved Mass Multiplication of Rhodiola crenulata Shoots Using Temporary Immersion Bioreactor with Forced Ventilation".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 166.6(2012):1480-1490. |
入库方式： OAI收割
来源：过程工程研究所
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