Human serum albumin stability and toxicity of anthraquinone dye alizarin complexone: An albumin-dye model
文献类型：期刊论文
| 作者 | Ding, Fei2,3; Zhang, Li4; Diao, Jian-Xiong1; Li, Xiu-Nan5; Ma, Lin6; Sun, Ying1 |
| 刊名 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| 出版日期 | 2012-05-01 |
| 卷号 | 79期号:0页码:238-246 |
| 关键词 | Anthraquinone dye Human serum albumin Circular dichroism Molecular modeling Fluorescence |
| ISSN号 | 0147-6513 |
| 通讯作者 | Sun, Y |
| 英文摘要 | The complexation between the primary vector of ligands in blood plasma, human serum albumin (HSA) and a toxic anthraquinone dye alizarin complexone, was unmasked by means of circular dichroism (CD), molecular modeling, steady state and time-resolved fluorescence, and UV/vis absorption measurements. The structural investigation of the complexed HSA through far-UV CD, three-dimensional and synchronous fluorescence shown the polypeptide chain of HSA partially destabilizing with a reduction of alpha-helix upon conjugation. From molecular modeling and competitive ligand binding results, Sudlow's site I, which was the same as that of warfarin-azapropazone site, was appointed to retain high-affinity for alizarin complexone. Moreover, steady state fluorescence displayed that static type and Forster energy transfer is the operational mechanism for the vanish in the tryptophan (Trp)-214 fluorescence, this corroborates time-resolved fluorescence that HSA-alizarin complexone adduct formation has an affinity of 10(5) M-1, and the driving forces were found to be chiefly pi-pi, hydrophobic, and hydrogen bonds, associated with an exothermic free energy change. These data should be utilized to illustrate the mechanism by which the toxicological action of anthraquinone dyes is mitigated by transporter HSA. (C) 2012 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Environmental Sciences ; Toxicology |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology |
| 关键词[WOS] | BETA-LACTOGLOBULIN ; WARFARIN-BINDING ; STEADY-STATE ; SITE-I ; PROTEIN ; FLUORESCENCE ; AFFINITY ; SPECIFICITY ; DERIVATIVES ; AZO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000302107000033 |
| 公开日期 | 2013-10-22 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3857]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China 2.China Agr Univ, Dept Chem, Beijing 100193, Peoples R China 3.MIT, Dept Biol Engn, Cambridge, MA 02139 USA 4.China Agr Univ, Dept Appl Chem, Minist Agr, Key Lab Pesticide Chem & Applicat Technol, Beijing 100193, Peoples R China 5.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 6.Tsinghua Univ, Sch Environm, Beijing 100084, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Fei,Zhang, Li,Diao, Jian-Xiong,et al. Human serum albumin stability and toxicity of anthraquinone dye alizarin complexone: An albumin-dye model[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2012,79(0):238-246. |
| APA | Ding, Fei,Zhang, Li,Diao, Jian-Xiong,Li, Xiu-Nan,Ma, Lin,&Sun, Ying.(2012).Human serum albumin stability and toxicity of anthraquinone dye alizarin complexone: An albumin-dye model.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,79(0),238-246. |
| MLA | Ding, Fei,et al."Human serum albumin stability and toxicity of anthraquinone dye alizarin complexone: An albumin-dye model".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 79.0(2012):238-246. |
入库方式： OAI收割
来源：过程工程研究所
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