Highly efficient synthesis of sucrose monolaurate by alkaline protease Protex 6L
文献类型：期刊论文
| 作者 | Wang, Xinran1,2; Miao, Shida1; Wang, Ping3,4; Zhang, Songping1 |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2012-04-01 |
| 卷号 | 109期号:0页码:7-12 |
| 关键词 | Sucrose monolaurate Protex 6L Response surface analysis Solvent mixture |
| ISSN号 | 0960-8524 |
| 通讯作者 | Zhang, SP |
| 英文摘要 | An alkaline protease from Bacillus licheniformis, Protex 6L, was used for synthesis of sucrose monolaurate from sucrose and vinyl laurate in a tert-amyl alcohol/DMSO/water solvent mixture. Introducing sucrose as powder after mixing vinyl laurate with solvent mixture resulted in a higher reaction rate than when sucrose was added as a solution in DMSO. Response surface methodology (RSM) was applied to optimize the major reaction variables, water content, temperature and pH of the lyophilized enzyme. The optimal conditions derived from RSM (3.4% water content, 43 degrees C and pH of 10.1) provided a high initial reaction rate of 8.66 +/- 0.3 mg/ml/h which agreed with the predicted value of 8.70 mg/ml/h. With 1.5 mg-enzyme/ml, 98.0% of the added sucrose was region-selectively converted to 1'-O-lauroylsucrose after 9 h. Under the optimized conditions, Protex 6L exhibited a higher productivity for sucrose ester synthesis than Novozym 435 and three other commonly used enzymes. (C) 2012 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | RESPONSE-SURFACE METHODOLOGY ; LIPASE-CATALYZED SYNTHESIS ; CANDIDA-ANTARCTICA LIPASE ; ENZYMATIC-SYNTHESIS ; SUGAR ESTERS ; REGIOSELECTIVE ACYLATION ; FRUCTOSE ESTERS ; WATER ACTIVITY ; SOLVENT ; PARAMETERS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301810500002 |
| 公开日期 | 2013-10-23 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3871]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA 4.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA |
| 推荐引用方式 GB/T 7714 | Wang, Xinran,Miao, Shida,Wang, Ping,et al. Highly efficient synthesis of sucrose monolaurate by alkaline protease Protex 6L[J]. BIORESOURCE TECHNOLOGY,2012,109(0):7-12. |
| APA | Wang, Xinran,Miao, Shida,Wang, Ping,&Zhang, Songping.(2012).Highly efficient synthesis of sucrose monolaurate by alkaline protease Protex 6L.BIORESOURCE TECHNOLOGY,109(0),7-12. |
| MLA | Wang, Xinran,et al."Highly efficient synthesis of sucrose monolaurate by alkaline protease Protex 6L".BIORESOURCE TECHNOLOGY 109.0(2012):7-12. |
入库方式： OAI收割
来源：过程工程研究所
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