Lipase immobilization on ionic liquid-modified magnetic nanoparticles: Ionic liquids controlled esters hydrolysis at oil-water interface
文献类型：期刊论文
| 作者 | Jiang, Yangyang1,2; Guo, Chen1; Gao, Hongshuai1; Xia, Hansong1; Mahmood, Iram1; Liu, Chunzhao1; Liu, Huizhou1 |
| 刊名 | AICHE JOURNAL
 |
| 出版日期 | 2012-04-01 |
| 卷号 | 58期号:4页码:1203-1211 |
| 关键词 | magnetic nanoparticles ionic liquids lipase oil-water two-phase ester hydrolysis |
| ISSN号 | 0001-1541 |
| 通讯作者 | Guo, C |
| 英文摘要 | Ester hydrolysis at oilwater interface by lipase covalently immobilized on ionic liquid-modified magnetic nanoparticles was investigated. Magnetic supports with a diameter of 1015 nm were synthesized by covalent binding of ionic liquids (chain length C4 and C8 and anions Cl-, BF4-, and PF6-) on the surface of Fe3O4 nanoparticles. Lipase was covalently immobilized on Fe3O4 nanoparticles using ionic liquids as the coupling reagent. Ionic liquid-modified magnetic nanoparticle-grafted lipase preferentially located at the oilwater interface. It has higher catalytic activity than its native counterpart. A modified MichaelisMenten model was used to elucidate the effect of stirring rate, aqueousorganic phase ratio, total amount of enzyme, and ester chain length. The influences of these conditions on esters hydrolysis at oilwater interface were consistent with the introduction of the ionic liquids interlayer. Ionic liquids could be used to control the oilwater interfacial characteristics during lipase catalyzed hydrolysis, and thus control the behavior of immobilized lipase. (C) 2011 American Institute of Chemical Engineers AIChE J, 2012 |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Chemical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | CANDIDA-RUGOSA LIPASE ; ENZYMATIC-HYDROLYSIS ; ORGANIC-SOLVENTS ; SILICA ; SYSTEMS ; CATALYSIS ; KINETICS ; DYE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301225700018 |
| 公开日期 | 2013-10-23 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3880]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing 100190, Peoples R China 2.Nanjing Chem Ind Grp, Res Inst, Nanjing 210048, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Yangyang,Guo, Chen,Gao, Hongshuai,et al. Lipase immobilization on ionic liquid-modified magnetic nanoparticles: Ionic liquids controlled esters hydrolysis at oil-water interface[J]. AICHE JOURNAL,2012,58(4):1203-1211. |
| APA | Jiang, Yangyang.,Guo, Chen.,Gao, Hongshuai.,Xia, Hansong.,Mahmood, Iram.,...&Liu, Huizhou.(2012).Lipase immobilization on ionic liquid-modified magnetic nanoparticles: Ionic liquids controlled esters hydrolysis at oil-water interface.AICHE JOURNAL,58(4),1203-1211. |
| MLA | Jiang, Yangyang,et al."Lipase immobilization on ionic liquid-modified magnetic nanoparticles: Ionic liquids controlled esters hydrolysis at oil-water interface".AICHE JOURNAL 58.4(2012):1203-1211. |
入库方式： OAI收割
来源：过程工程研究所
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