Improved cardenolide production in Calotropis gigantea hairy roots using mechanical wounding and elicitation
文献类型：期刊论文
| 作者 | Sun, Jian1,2; Xiao, Jie1,2; Wang, Xiaodong1; Yuan, Xiaofan1; Zhao, Bing1 |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2012-03-01 |
| 卷号 | 34期号:3页码:563-569 |
| 关键词 | Cardenolides Calotropis gigantea Hairy root cultures Mechanical wounding Elicitation |
| ISSN号 | 0141-5492 |
| 通讯作者 | Zhao, B |
| 英文摘要 | A hairy root culture system of Calotropis gigantea was established and effects of mechanical wounding (MW) and elicitors [methyl jasmonate (MJ), yeast extract (YE) and chitosan (CS)] on cardenolide production were investigated. All treatments stimulated the production of cardenolide in hairy root cultures of C. gigantea. CS was the most effective elicitor, followed by MJ. YE and MW also improved cardenolide yield in individual treatments. The highest cardenolide yield (1,050 +/- 55 mg/l) was obtained after adding 50 mg CS/l for 20 days, which was 2.7-fold higher than the control. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | CARDIAC-GLYCOSIDES ; CULTURES ; INDUCTION ; LEAVES ; PLANT ; LATEX ; BR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301296300022 |
| 公开日期 | 2013-10-23 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3883]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Jian,Xiao, Jie,Wang, Xiaodong,et al. Improved cardenolide production in Calotropis gigantea hairy roots using mechanical wounding and elicitation[J]. BIOTECHNOLOGY LETTERS,2012,34(3):563-569. |
| APA | Sun, Jian,Xiao, Jie,Wang, Xiaodong,Yuan, Xiaofan,&Zhao, Bing.(2012).Improved cardenolide production in Calotropis gigantea hairy roots using mechanical wounding and elicitation.BIOTECHNOLOGY LETTERS,34(3),563-569. |
| MLA | Sun, Jian,et al."Improved cardenolide production in Calotropis gigantea hairy roots using mechanical wounding and elicitation".BIOTECHNOLOGY LETTERS 34.3(2012):563-569. |
入库方式： OAI收割
来源：过程工程研究所
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