The orchestration of cellular and humoral responses is facilitated by divergent intracellular antigen trafficking in nanoparticle-based therapeutic vaccine
文献类型：期刊论文
| 作者 | Yue, Hua1,2; Wei, Wei1; Fan, Bei3; Yue, Zhanguo1,2; Wang, Lianyan1; Ma, Guanghui1; Su, Zhiguo1 |
| 刊名 | PHARMACOLOGICAL RESEARCH
 |
| 出版日期 | 2012-02-01 |
| 卷号 | 65期号:2页码:189-197 |
| ISSN号 | 1043-6618 |
| 关键词 | Cellular immune response Humoral immune response Intracellular trafficking Polylactide nanoparticles Therapeutic vaccine |
| 通讯作者 | Ma, GH |
| 英文摘要 | Therapeutic vaccination for the treatment of chronic hepatitis B is promising but has so far shown limited clinical efficacy. Herein, we employ polylactide nanoparticles (NPs) as the vaccine adjuvant and systematically explore their effect on activation of specific immunity and the underlying theoretical mechanisms. In vitro studies show that hepatitis B surface antigen (HBsAg) accumulates in antigen-presenting cells (APCs) to a larger content (270%) with the assistant of NP in comparison with the pure-antigen group. Besides the elevated costimulators (CD80/86) and increased major histocompatibility complex (MHC) II molecules, the MHC I molecules are also found upregulated. This result is mostly owing to the divergent antigen trafficking ways of NP-antigen in APCs, especially for the escape of exogenous HBsAg from the lysosomes to the cytosol. Interestingly, the MHC I level is downregulated in alum-antigen group, indicating a possible reason for its inefficiency in priming cellular response. Further in vivo experiments establish that NP-antigen group indeed enhances the CD8(+) CTL cytotoxicity and IFN-gamma cytokine secretion. Meanwhile, specific antibody titer is also upregulated, and even surpasses that of the commercialized alum-antigen. All these results strongly support that NP-based antigen promotes an orchestration of cellular and humoral immune response, exhibiting favorable intrinsic properties to be applied in therapeutic vaccines. (C) 2011 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Pharmacology & Pharmacy |
| 研究领域[WOS] | Pharmacology & Pharmacy |
| 关键词[WOS] | CHRONIC HEPATITIS-B ; PREMIX MEMBRANE EMULSIFICATION ; IMMUNE-RESPONSES ; DENDRITIC CELLS ; GOLD NANOPARTICLES ; ALUMINUM ADJUVANTS ; CROSS-PRESENTATION ; VIRAL-HEPATITIS ; VIRUS INFECTION ; T-CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301868300006 |
| 公开日期 | 2013-10-23 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3886]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Hualan Biol Engn Inc, Xinxiang 453003, Henan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yue, Hua,Wei, Wei,Fan, Bei,et al. The orchestration of cellular and humoral responses is facilitated by divergent intracellular antigen trafficking in nanoparticle-based therapeutic vaccine[J]. PHARMACOLOGICAL RESEARCH,2012,65(2):189-197. |
| APA | Yue, Hua.,Wei, Wei.,Fan, Bei.,Yue, Zhanguo.,Wang, Lianyan.,...&Su, Zhiguo.(2012).The orchestration of cellular and humoral responses is facilitated by divergent intracellular antigen trafficking in nanoparticle-based therapeutic vaccine.PHARMACOLOGICAL RESEARCH,65(2),189-197. |
| MLA | Yue, Hua,et al."The orchestration of cellular and humoral responses is facilitated by divergent intracellular antigen trafficking in nanoparticle-based therapeutic vaccine".PHARMACOLOGICAL RESEARCH 65.2(2012):189-197. |
入库方式： OAI收割
来源：过程工程研究所
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