Potential toxicity and affinity of triphenylmethane dye malachite green to lysozyme
文献类型：期刊论文
| 作者 | Ding, Fei1,2; Li, Xiu-Nan3; Diao, Jian-Xiong4; Sun, Ye1; Zhang, Li5; Ma, Lin6; Yang, Xin-Ling1; Zhang, Li1; Sun, Ying4 |
| 刊名 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| 出版日期 | 2012-04-01 |
| 卷号 | 78期号:0页码:41-49 |
| 关键词 | Malachite green Lysozyme Molecular modeling Fluorescence spectroscopy Circular dichroism |
| ISSN号 | 0147-6513 |
| 通讯作者 | Zhang, L |
| 英文摘要 | Malachite green is a triphenylmethane dye that is used extensively in many industrial and aquacultural processes, generating environmental concerns and health problems to human being. In this contribution, the complexation between lysozyme and malachite green was verified by means of computer-aided molecular modeling, steady state and time-resolved fluorescence, and circular dichroism (CD) approaches. The precise binding patch of malachite green in lysozyme has been identified from molecular modeling and ANS displacement, Trp-62, Trp-63, and Trp-108 residues of lysozyme were earmarked to possess high-affinity for this dye, the principal forces in the lysozyme-malachite green adduct are hydrophobic and pi-pi interactions. Steady state fluorescence proclaimed the complex of malachite green with lysozyme yields quenching through static type, which substantiates time-resolved fluorescence measurements that lysozyme-malachite green conjugation formation has an affinity of 10(3) M-1. Moreover, via molecular modeling and also CD data, we can safely arrive at a conclusion that the polypeptide chain of lysozyme partially destabilized upon complexation with malachite green. The data emerged here will help to further understand the toxicological action of malachite green in human body. (C) 2012 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Environmental Sciences ; Toxicology |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology |
| 关键词[WOS] | HUMAN SERUM-ALBUMIN ; HAMSTER EMBRYO CELLS ; CIRCULAR-DICHROISM SPECTRA ; FED LEUCOMALACHITE GREEN ; EGG-WHITE LYSOZYME ; LIGAND-BINDING ; B6C3F(1) MICE ; FLUORESCENCE SPECTROSCOPY ; BETA-LACTOGLOBULIN ; RATS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301316500007 |
| 公开日期 | 2013-10-23 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3887]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.China Agr Univ, Dept Chem, Beijing 100193, Peoples R China 2.MIT, Dept Biol Engn, Cambridge, MA 02139 USA 3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 4.China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China 5.China Agr Univ, Dept Appl Chem, Minist Agr, Key Lab Pesticide Chem & Applicat Technol, Beijing 100193, Peoples R China 6.Tsinghua Univ, Sch Environm, Beijing 100084, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Fei,Li, Xiu-Nan,Diao, Jian-Xiong,et al. Potential toxicity and affinity of triphenylmethane dye malachite green to lysozyme[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2012,78(0):41-49. |
| APA | Ding, Fei.,Li, Xiu-Nan.,Diao, Jian-Xiong.,Sun, Ye.,Zhang, Li.,...&Sun, Ying.(2012).Potential toxicity and affinity of triphenylmethane dye malachite green to lysozyme.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,78(0),41-49. |
| MLA | Ding, Fei,et al."Potential toxicity and affinity of triphenylmethane dye malachite green to lysozyme".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 78.0(2012):41-49. |
入库方式： OAI收割
来源：过程工程研究所
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