Novel in situ product removal technique for simultaneous production of propionic acid and vitamin B12 by expanded bed adsorption bioreactor
文献类型：期刊论文
| 作者 | Wang, Peng; Wang, Yunshan1; Liu, Yongdong; Shi, Hong; Su, Zhiguo |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 104期号:0页码:652-659 |
| 关键词 | Propionibacterium freudenreichii Propionic acid Vitamin B12 In situ product removal Expanded bed |
| ISSN号 | 0960-8524 |
| 通讯作者 | Wang, YS |
| 英文摘要 | A new type of in situ product removal (ISPR) technique of expanded bed adsorption (EBA) bioreactor was studied to simultaneously produce extracellular propionic acid and intracellular vitamin B12 by Propionibacterium freudenreichii CICC 10019. Resin screening experiments showed that the ZGA330 resin have the best biocompatibility and highest adsorption for propionic acid. Through the EBA bioreactor, propionic acid could be recovered efficiently by semi-continuous recirculation of the unfiltered broth, which eliminated the feedback inhibition of propionic acid. Fed-batch fermentation was carried out using the EBA system, resulting in a propionic acid concentration of 52.5 g L-1 and vitamin B12 concentration of 43.04 mg L-1 at 160h, which correspond to product yields of 0.66 g g(-1) and 0.54 mg g(-1), respectively. The present study suggests that the EBA bioreactor can be utilized for the simple and economical production of propionic acid and vitamin B12 in a single fermentation process. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | ION-EXCHANGE RESIN ; PROPIONIBACTERIUM-ACIDIPROPIONICI ; BATCH FERMENTATION ; LACTIC-ACID ; RECOVERY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301155800088 |
| 公开日期 | 2013-10-23 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3908]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Peng,Wang, Yunshan,Liu, Yongdong,et al. Novel in situ product removal technique for simultaneous production of propionic acid and vitamin B12 by expanded bed adsorption bioreactor[J]. BIORESOURCE TECHNOLOGY,2012,104(0):652-659. |
| APA | Wang, Peng,Wang, Yunshan,Liu, Yongdong,Shi, Hong,&Su, Zhiguo.(2012).Novel in situ product removal technique for simultaneous production of propionic acid and vitamin B12 by expanded bed adsorption bioreactor.BIORESOURCE TECHNOLOGY,104(0),652-659. |
| MLA | Wang, Peng,et al."Novel in situ product removal technique for simultaneous production of propionic acid and vitamin B12 by expanded bed adsorption bioreactor".BIORESOURCE TECHNOLOGY 104.0(2012):652-659. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。