Functional gigaporous polystyrene microspheres facilitating separation of poly(ethylene glycol)-protein conjugate
文献类型：期刊论文
| 作者 | Zhai, Yanqin1,2; Zhou, Weiqing1; Wei, Wei1; Qu, Jianbo3; Lei, Jiandu1; Su, Zhiguo1; Ma, Guanghui1 |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2012-01-27 |
| 卷号 | 712期号:0页码:152-161 |
| 关键词 | Gigaporous Polystyrene microspheres Poly(ethylene glycol)-protein Chromatography media |
| ISSN号 | 0003-2670 |
| 通讯作者 | Lei, JD |
| 英文摘要 | A novel sulfopropyl gigaporous polystyrene (SP-GP) microsphere enhancing the separation of poly(ethylene glycol)-protein (PEGylated protein) was first presented. The SP-GP microspheres were successfully prepared by introducing sulfopropyl groups into agarose-coated gigaporous polystyrene microspheres and used as chromatography media. Compared with a commercial medium, SP-GP microspheres exhibited improved column efficiency and reduced backpressure with increasing flow velocity, which could ensure its use in high-speed chromatography. Furthermore, a higher protein recovery and purity of the PEGylated protein could be obtained, even when SP-GP was applied at a flow velocity of 1224 cm h(-1). Additionally, the dynamic binding capacity (DBC) of SP-GP was significantly improved, which was higher than 10 mg mL(-1) medium even at a flow velocity of 306 cm h(-1). Further investigation using a laser scanning confocal microscope (LSCM) demonstrated that the static adsorption equilibrium of the PEGylated protein on SP-GP could be completed in 5 min, whereas a much longer period (ca. 60 min) was required for the commercial medium, indicating that the mass transfer of SP-GP was much faster with the gigaporous structure. All of these results strongly support that our developed SP-GP could serve as a promising cation exchange chromatography resin for high-speed separation, especially for biomolecules of high molecular weight. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | ION-EXCHANGE CHROMATOGRAPHY ; LASER-SCANNING MICROSCOPY ; PEGYLATED PROTEINS ; MEDIA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000300764700020 |
| 公开日期 | 2013-10-25 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4287]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.SinoCellTech Ltd, Purificat Proc Dev, Beijing 100176, Peoples R China 3.China Univ Petr, Ctr Bioengn & Biotechnol, Qingdao 266555, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhai, Yanqin,Zhou, Weiqing,Wei, Wei,et al. Functional gigaporous polystyrene microspheres facilitating separation of poly(ethylene glycol)-protein conjugate[J]. ANALYTICA CHIMICA ACTA,2012,712(0):152-161. |
| APA | Zhai, Yanqin.,Zhou, Weiqing.,Wei, Wei.,Qu, Jianbo.,Lei, Jiandu.,...&Ma, Guanghui.(2012).Functional gigaporous polystyrene microspheres facilitating separation of poly(ethylene glycol)-protein conjugate.ANALYTICA CHIMICA ACTA,712(0),152-161. |
| MLA | Zhai, Yanqin,et al."Functional gigaporous polystyrene microspheres facilitating separation of poly(ethylene glycol)-protein conjugate".ANALYTICA CHIMICA ACTA 712.0(2012):152-161. |
入库方式： OAI收割
来源：过程工程研究所
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