Features of the complex of food additive hesperidin to hemoglobin
文献类型：期刊论文
| 作者 | Ding, Fei2,3; Sun, Ye2; Diao, Jian-Xiong1; Li, Xiu-Nan4; Yang, Xin-Ling2; Sun, Ying1; Zhang, Li5 |
| 刊名 | JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
 |
| 出版日期 | 2012-01-05 |
| 卷号 | 106期号:0页码:53-60 |
| 关键词 | Flavonoid Hesperidin Hemoglobin Molecular modeling Fluorescence Circular dichroism |
| ISSN号 | 1011-1344 |
| 通讯作者 | Sun, Y |
| 英文摘要 | The purpose of the current work was to examine the complexation of a mammalian protein, hemoglobin (Hb) with a food additive hesperidin at physiological conditions. Molecular modeling, fluorescence, and circular dichroism (CD) methods were exploited to analyze the binding domain, affinity, and the effects of hesperidin conjugation on Hb spatial structure. From molecular modeling, central cavity of Hb was assigned to retain high-affinity for hesperidin, this corroborates the steady state fluorescence and hydrophobic ANS probe results. The association of hesperidin with Hb emerges fluorescence quenching via static type, this phenomenon display that the ground state complex formation with an affinity of 10(4) M-1, and hypsochromic effect transpires. Additionally, the alterations of synchronous fluorescence, CD, and three-dimensional fluorescence suggest that the polypeptide chain of Hb partially folding after conjugation with hesperidin. The above data suggest that Hb plays a significant role in the plasma distribution and transportation of hesperidin and related dietary flavonoids. (C) 2011 Published by Elsevier B.V. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | BOVINE SERUM-ALBUMIN ; PLASMA-PROTEINS ; LIGAND-BINDING ; X-RAY ; FLAVONOIDS ; THERMODYNAMICS ; OXYHEMOGLOBIN ; EMISSION ; SPECTRA ; SITE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000300387800007 |
| 公开日期 | 2013-10-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4300]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China 2.China Agr Univ, Dept Chem, Beijing, Peoples R China 3.MIT, Dept Biol Engn, Cambridge, MA 02139 USA 4.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 5.China Agr Univ, Dept Appl Chem, Minist Agr, Key Lab Pesticide Chem & Applicat Technol, Beijing 100193, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Fei,Sun, Ye,Diao, Jian-Xiong,et al. Features of the complex of food additive hesperidin to hemoglobin[J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,2012,106(0):53-60. |
| APA | Ding, Fei.,Sun, Ye.,Diao, Jian-Xiong.,Li, Xiu-Nan.,Yang, Xin-Ling.,...&Zhang, Li.(2012).Features of the complex of food additive hesperidin to hemoglobin.JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,106(0),53-60. |
| MLA | Ding, Fei,et al."Features of the complex of food additive hesperidin to hemoglobin".JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 106.0(2012):53-60. |
入库方式： OAI收割
来源：过程工程研究所
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