Manipulation of heterogeneity product in 4'-demethylepipodophyllotoxin biotransformation process by using yeast extract as nitrogen source
文献类型：期刊论文
| 作者 | Zhao, Wei1,2; Li, Hong-Mei1,2; Wan, Duan-Ji1; Tang, Ya-Jie1,2,3,4 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 93期号:1页码:107-115 |
| 关键词 | Biotransformation product heterogeneity Biotransformation metabolic pathway Yeast extract 4 '-Demethylepipodophyllotoxin Alternaria alternata S-f6 |
| ISSN号 | 0175-7598 |
| 通讯作者 | Tang, YJ |
| 英文摘要 | Manipulation of product heterogeneity was attempted by using yeast extract as nitrogen source in Alternaria alternata S-f6 transformation process of 4'-demethylepipodophyllotoxin. When the nitrogen source of NaNO(3) was replaced by yeast extract, the heterogeneity of biotransformation products was significantly varied from a single product (i.e., 4'-demethylpodophyllotoxone) to four podophyllum derivates. According to the kinetics of 4'-demethylepipodophyllotoxin biotransformation process by A. alternata S-f6, the starting substrate of 4'-demethylepipodophyllotoxin was preferentially transformed to produce 4'-demethylpodophyllotoxone (1) with an oxidation reaction. By the further comparison of products configuration, 4 beta-caprinoyl-4'-demethylepipodophyllotoxin (3) was produced from 4'-demethylpodophyllotoxone (1) instead of 4'-demethylisopicropodophyllone (2), which might be produced from 4'-demethylpodophyllotoxone (1) with the isomerization of lactone. Finally, 4'-demethylisopicropodophyllone (2) was hydrolyzed to produce 3 alpha-hydroxymethyl-(6, 7)-dioxol-4-one-naphthalene (4). This work shows new information on the 4'-demethylepipodophyllotoxin biotransformation process by A. alternata S-f6 and provides a foundation for further studies on the structural diversification of a bioactive natural lead compound. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | TRUFFLE TUBER-SINENSE ; POLYSACCHARIDES PRODUCTION ; CELL-GROWTH ; PODOPHYLLOTOXIN ; CULTURE ; 4'-DEMETHYLEPIPODOPHYLLOTOXIN ; BIOSYNTHESIS ; CARBON ; ACID |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000298853600010 |
| 公开日期 | 2013-10-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4320]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Hubei Univ Technol, Coll Bioengn, Hubei Prov Key Lab Ind Microbiol, Key Lab Fermentat Engn,Minist Educ, Wuhan 430068, Peoples R China 2.Chinese Acad Sci, Shanghai Adv Res Inst, Lab Biorefinery Engn, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 4.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Wei,Li, Hong-Mei,Wan, Duan-Ji,et al. Manipulation of heterogeneity product in 4'-demethylepipodophyllotoxin biotransformation process by using yeast extract as nitrogen source[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2012,93(1):107-115. |
| APA | Zhao, Wei,Li, Hong-Mei,Wan, Duan-Ji,&Tang, Ya-Jie.(2012).Manipulation of heterogeneity product in 4'-demethylepipodophyllotoxin biotransformation process by using yeast extract as nitrogen source.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,93(1),107-115. |
| MLA | Zhao, Wei,et al."Manipulation of heterogeneity product in 4'-demethylepipodophyllotoxin biotransformation process by using yeast extract as nitrogen source".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 93.1(2012):107-115. |
入库方式： OAI收割
来源：过程工程研究所
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