Deliberate manipulation of the surface hydrophobicity of an adsorbent for an efficient purification of a giant molecule with multiple subunits
文献类型：期刊论文
| 作者 | Zhao, Lan1,2; Liu, Yongdong1; Huang, Yongdong1; Li, Xiunan1; Wang, Yinjue1; Li, Yan1; Ma, Guanghui1; Su, Zhiguo1 |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2011-11-01 |
| 卷号 | 34期号:22页码:3186-3193 |
| 关键词 | Hydrophobic interaction chromatography Ligand density Purification rHBsAg Spacer arm |
| ISSN号 | 1615-9306 |
| 通讯作者 | Su, ZG |
| 英文摘要 | Hydrophobic interaction chromatography (HIC) is often an inevitable step for a satisfying purification in giant vaccine molecules production. But great mass and activity loss associated with poor purity often occur simultaneously. In this paper, high purity and high bioactivity recovery for the HIC process of hepatitis B surface antigen (rHBsAg) purification were achieved through manipulation of surface hydrophobicity of the adsorbent. Spacer arm length and ligand density were regulated, respectively, through which the interaction between the vaccine and the adsorbent was manipulated deliberately. It was found even in a narrow scope, varying spacer arm length and ligand density resulted in purification factor changing from 1 to 96.5, and rHBsAg recovery from 3 to 91%. The optimal purification performance was achieved when the spacer arm was C8 and the ligand density was 9.2 mu mol/g suction-dried wet gel with an average distance of ligands of 3.6 nm. This deliberate regulation strategy represents a new approach of improving purification of giant multi-subunit proteins. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | LASER-SCANNING MICROSCOPY ; CHINESE-HAMSTER OVARY ; HEPATITIS-B VACCINES ; HANSENULA-POLYMORPHA ; PICHIA-PASTORIS ; LIGAND DENSITY ; SPACER ARM ; ANTIGEN ; RECOMBINANT ; CHROMATOGRAPHY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000297949400010 |
| 公开日期 | 2013-10-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4339]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Lan,Liu, Yongdong,Huang, Yongdong,et al. Deliberate manipulation of the surface hydrophobicity of an adsorbent for an efficient purification of a giant molecule with multiple subunits[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(22):3186-3193. |
| APA | Zhao, Lan.,Liu, Yongdong.,Huang, Yongdong.,Li, Xiunan.,Wang, Yinjue.,...&Su, Zhiguo.(2011).Deliberate manipulation of the surface hydrophobicity of an adsorbent for an efficient purification of a giant molecule with multiple subunits.JOURNAL OF SEPARATION SCIENCE,34(22),3186-3193. |
| MLA | Zhao, Lan,et al."Deliberate manipulation of the surface hydrophobicity of an adsorbent for an efficient purification of a giant molecule with multiple subunits".JOURNAL OF SEPARATION SCIENCE 34.22(2011):3186-3193. |
入库方式： OAI收割
来源：过程工程研究所
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