Enhanced biodesulfurization by expression of dibenzothiophene uptake genes in Rhodococcus erythropolis
文献类型：期刊论文
| 作者 | Wang, Ze-long1,2; Wang, Dan1,2; Li, Qiang1; Li, Wang-liang1; Tang, Huang1,2; Xing, Jian-min1 |
| 刊名 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2011-09-01 |
| 卷号 | 27期号:9页码:1965-1970 |
| 关键词 | Biodesulfurization Dibenzothiophene uptake Cell lysate HcuABC |
| ISSN号 | 0959-3993 |
| 通讯作者 | Xing, JM |
| 英文摘要 | The transfer of dibenzothiophene (DBT) and its derivatives into cells is a critical step for biodesulfurization. The desulfurization reactions of resting cells and cell lysate were studied, which showed that the desulfurization rate of DBT, especially 4, 6-dimethyldibenzothiophene (4, 6-DMDBT) in Rhodococcus erythropolis LSSE8-1 was seriously affected by the transfer into cells. The inhibited effect of NaN(3) on desulfurization reactions was studied, which confirmed that the transfer of DBT into cells was an active transport in R. erythropolis LSSE8-1. The uptake-genes of DBT and its derivatives (HcuABC) of Pseudomonas delafieldii R-8 were introduced into the specific desulfurization bacterium, R. erythropolis LSSE8-1. Compared with the wild type, the strains bearing HcuABC genes showed a higher desulfurization activity. The desulfurization ratio of DBT showed a 19% increase, and 13% increase of 4, 6-DMDBT. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | FOSSIL-FUELS ; N-TETRADECANE ; SYSTEMS ; CELLS ; DESULFURIZATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000293793900001 |
| 公开日期 | 2013-10-31 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4646]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Gard Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ze-long,Wang, Dan,Li, Qiang,et al. Enhanced biodesulfurization by expression of dibenzothiophene uptake genes in Rhodococcus erythropolis[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2011,27(9):1965-1970. |
| APA | Wang, Ze-long,Wang, Dan,Li, Qiang,Li, Wang-liang,Tang, Huang,&Xing, Jian-min.(2011).Enhanced biodesulfurization by expression of dibenzothiophene uptake genes in Rhodococcus erythropolis.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,27(9),1965-1970. |
| MLA | Wang, Ze-long,et al."Enhanced biodesulfurization by expression of dibenzothiophene uptake genes in Rhodococcus erythropolis".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 27.9(2011):1965-1970. |
入库方式： OAI收割
来源：过程工程研究所
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