Effect of molecular mobility on coupled enzymatic reactions involving cofactor regeneration using nanoparticle-attached enzymes
文献类型：期刊论文
| 作者 | Zheng, Muqing3,4; Zhang, Songping3; Ma, Guanghui3; Wang, Ping1,2 |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2011-07-20 |
| 卷号 | 154期号:4页码:274-280 |
| 关键词 | Enzyme Superparamagnetic nanoparticles Cofactor regeneration Biotransformation Biosynthesis |
| ISSN号 | 0168-1656 |
| 通讯作者 | Wang, P |
| 英文摘要 | Cofactor-dependent multi-step enzymatic reactions generally require dynamic interactions among cofactor, enzyme and substrate molecules. Maintaining such molecular interactions can be quite challenging especially when the catalysts are tethered to solid state supports for heterogeneous catalysis for either biosynthesis or biosensing. The current work examines the effects of the pattern of immobilization, which presumably impacts molecular interactions on the surface of solid supports, on the reaction kinetics of a multienzymic system including glutamate dehydrogenase, glucose dehydrogenase and cofactor NAD(H). Interestingly, particle collision due to Brownian motion of nanoparticles successfully enabled the coupled reactions involving a regeneration cycle of NAD(H) even when the enzymes and cofactor were immobilized separately onto superparamagnetic nanoparticles (124 nm). The impact of particle motion and collision was evident in that the overall reaction rate was increased by over 100% by applying a moderate alternating magnetic field (500 Hz, 17 Gs), or using additional spacers, both of which could improve the mobility of the immobilized catalysts. We further observed that integrated immobilization, which allowed the cofactor to be placed in the molecular vicinity of enzymes on the same nanoparticles, could enhance the reaction rate by 1.8 fold. These results demonstrated the feasibility in manipulating molecular interactions among immobilized catalyst components by using nanoscale fabrication for efficient multienzymic biosynthesis. (C) 2011 Elsevier B. V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | COLLOIDAL SILICA COATINGS ; MAGNETIC NANOPARTICLES ; IMMOBILIZED ENZYMES ; NADH REGENERATION ; HIGH-PERFORMANCE ; BIOCATALYSIS ; LIPASE ; DEHYDROGENASES ; PARTICLES ; BINDING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000292958600013 |
| 公开日期 | 2013-10-31 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4649]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA 2.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA 3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Muqing,Zhang, Songping,Ma, Guanghui,et al. Effect of molecular mobility on coupled enzymatic reactions involving cofactor regeneration using nanoparticle-attached enzymes[J]. JOURNAL OF BIOTECHNOLOGY,2011,154(4):274-280. |
| APA | Zheng, Muqing,Zhang, Songping,Ma, Guanghui,&Wang, Ping.(2011).Effect of molecular mobility on coupled enzymatic reactions involving cofactor regeneration using nanoparticle-attached enzymes.JOURNAL OF BIOTECHNOLOGY,154(4),274-280. |
| MLA | Zheng, Muqing,et al."Effect of molecular mobility on coupled enzymatic reactions involving cofactor regeneration using nanoparticle-attached enzymes".JOURNAL OF BIOTECHNOLOGY 154.4(2011):274-280. |
入库方式： OAI收割
来源：过程工程研究所
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