mPEG-PLA microspheres with narrow size distribution increase the controlled release effect of recombinant human growth hormone
文献类型：期刊论文
| 作者 | Wei, Yi1,2; Wang, Yu-Xia1; Wang, Wei3; Ho, Sa V.3; Wei, Wei1,2; Ma, Guang-Hui1 |
| 刊名 | JOURNAL OF MATERIALS CHEMISTRY
 |
| 出版日期 | 2011 |
| 卷号 | 21期号:34页码:12691-12699 |
| 关键词 | POLY-DL-LACTIDE-POLY(ETHYLENE GLYCOL) MICROSPHERES SUSTAINED-RELEASE ACID) MICROPARTICLES PROCESS PARAMETERS PLGA MICROSPHERES STABILITY PROTEINS DELIVERY FORMULATION MICROENCAPSULATION |
| ISSN号 | 0959-9428 |
| 通讯作者 | Wang, YX |
| 英文摘要 | The overall goal of this research is to prepare amphiphilic poly(monomethoxypolyethylene glycol-co-D,L-lactide) (mPEG-PLA, PELA) microspheres with narrow size distribution for sustained release of recombinant human growth hormone (rhGH) without any exogenous stabilizing excipients. In order to obtain microspheres with narrow size distribution, we used a double emulsion method combined with a premix membrane emulsification technique. The morphology, internal structure, rhGH encapsulation efficiency, in vitro release and rhGH stability of PELA microspheres were characterized in detail. The results were compared with those of rhGH encapsulated in poly(D, L-lactic acid) (PLA) and poly(D, L-lactic-co-glycolic acid) (PLGA) microspheres. All microspheres possessed a narrow size distribution and had mean diameters of 2 mm. The encapsulation efficiency of PELA, PLA, and PLGA microspheres were 89.3%, 65.0% and 58.6%, respectively. PELA microspheres showed an initial burst release of 14.2% rhGH, followed by a constant release of 78.3% over a 45-day period. Whereas, the PLA and PLGA microspheres showed higher burst levels of 22.3% and 26.2%, followed by release of 49.1% and 60.5%, respectively. In addition, the PELA microspheres maintained much better integrity of rhGH than PLA and PLGA microspheres. These results suggested that PELA is an excellent polymer for encapsulating rhGH. We propose that the mPEG-PLA block copolymer acts in a similar way to a surfactant and that it may orientate itself on the biphasic interface, thereby minimizing the contact of protein with the oil/water interface for protein stabilization. Furthermore, the microcosmic mechanisms responsible for rhGH encapsulation and rhGH stability were elucidated using a confocal laser scanning microscope. These results are promising for the construction of a sustained release system for rhGH using PELA microspheres. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| 类目[WOS] | Chemistry, Physical ; Materials Science, Multidisciplinary |
| 研究领域[WOS] | Chemistry ; Materials Science |
| 关键词[WOS] | POLY-DL-LACTIDE-POLY(ETHYLENE GLYCOL) MICROSPHERES ; SUSTAINED-RELEASE ; ACID) MICROPARTICLES ; PROCESS PARAMETERS ; PLGA MICROSPHERES ; STABILITY ; PROTEINS ; DELIVERY ; FORMULATION ; MICROENCAPSULATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000293898300019 |
| 公开日期 | 2013-10-31 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4650]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100190, Peoples R China 3.Pfizer Inc, BioTherapeut R&D, Chesterfield, MO 63017 USA |
| 推荐引用方式 GB/T 7714 | Wei, Yi,Wang, Yu-Xia,Wang, Wei,et al. mPEG-PLA microspheres with narrow size distribution increase the controlled release effect of recombinant human growth hormone[J]. JOURNAL OF MATERIALS CHEMISTRY,2011,21(34):12691-12699. |
| APA | Wei, Yi,Wang, Yu-Xia,Wang, Wei,Ho, Sa V.,Wei, Wei,&Ma, Guang-Hui.(2011).mPEG-PLA microspheres with narrow size distribution increase the controlled release effect of recombinant human growth hormone.JOURNAL OF MATERIALS CHEMISTRY,21(34),12691-12699. |
| MLA | Wei, Yi,et al."mPEG-PLA microspheres with narrow size distribution increase the controlled release effect of recombinant human growth hormone".JOURNAL OF MATERIALS CHEMISTRY 21.34(2011):12691-12699. |
入库方式： OAI收割
来源：过程工程研究所
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