Sucrose monolaurate synthesis with Protex 6L immobilized on electrospun TiO2 nanofiber
文献类型：期刊论文
| 作者 | Wang, Xin-Ran1,2; Zhang, Song-Ping1; Wang, Ping3,4 |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2011-09-01 |
| 卷号 | 33期号:9页码:1831-1835 |
| 关键词 | Protex 6L Sucrose monolaurate TiO2 nanofiber Enzyme immobilization |
| ISSN号 | 0141-5492 |
| 通讯作者 | Zhang, SP |
| 英文摘要 | TiO2 nanofibers with uniform diameter about 125 nm were prepared based on sol-gel process and electrospinning technology. Protex 6L, an industrial alkaline protease, was covalently immobilized on TiO2 nanofiber through gamma-aminopropyltriethoxysilane modification and glutaraldehyde crosslinking. With 2 (v/v)% glutaraldehyde as crosslinker, the enzyme loading is about 201 mg (g nanofiber membrane)(-1), and the specific activity of the immobilized Protex 6L is 2.45 mu mol h(-1) ml(-1) mg(-1) protein for synthesis of sucrose monolaurate from sucrose and vinyl laurate. The optimal condition for sucrose monolaurate production is 5% (v/v) water content in DMSO/2-methyl-2-butanol solvent mixture and 50A degrees C. Under this condition, 97% conversion was achieved within 36 h by nanofibrous Protex 6L, which is corresponding to a productivity 34 times higher than that of most widely used Novozym 435. After 10 cycles reuse, nanofibrous Protex 6L retained 52.4% of its original activity. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | DISACCHARIDE ; ENZYME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000293752700018 |
| 公开日期 | 2013-11-01 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4686]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA 4.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA |
| 推荐引用方式 GB/T 7714 | Wang, Xin-Ran,Zhang, Song-Ping,Wang, Ping. Sucrose monolaurate synthesis with Protex 6L immobilized on electrospun TiO2 nanofiber[J]. BIOTECHNOLOGY LETTERS,2011,33(9):1831-1835. |
| APA | Wang, Xin-Ran,Zhang, Song-Ping,&Wang, Ping.(2011).Sucrose monolaurate synthesis with Protex 6L immobilized on electrospun TiO2 nanofiber.BIOTECHNOLOGY LETTERS,33(9),1831-1835. |
| MLA | Wang, Xin-Ran,et al."Sucrose monolaurate synthesis with Protex 6L immobilized on electrospun TiO2 nanofiber".BIOTECHNOLOGY LETTERS 33.9(2011):1831-1835. |
入库方式： OAI收割
来源：过程工程研究所
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