Surface Charge Affects Cellular Uptake and Intracellular Trafficking of Chitosan-Based Nanoparticles
文献类型：期刊论文
| 作者 | Yue, Zhan-Guo1,2; Wei, Wei1; Lv, Pi-Ping1,2; Yue, Hua1,2; Wang, Lian-Yan1; Su, Zhi-Guo1; Ma, Guang-Hui1 |
| 刊名 | BIOMACROMOLECULES
 |
| 出版日期 | 2011-07-01 |
| 卷号 | 12期号:7页码:2440-2446 |
| 关键词 | MEMBRANE EMULSIFICATION TECHNIQUE MACROMOLECULAR THERAPEUTICS QUATERNIZED CHITOSAN GOLD NANOPARTICLES DELIVERY CARRIER DESIGN DRUG HYDROGELS MICROSPHERES |
| ISSN号 | 1525-7797 |
| 通讯作者 | Ma, GH |
| 英文摘要 | Chitosan-based nanoparticles (NPs) are widely used in drug delivery, device-based therapy, tissue engineering, and medical imaging. In this aspect, a clear understanding of how physicochemical properties of these NPs affect the cytological response is in high demand. The objective of this study is to evaluate the effect of surface charge on cellular uptake profiles (rate and amount) and intracellular trafficking. We fabricate three kinds of NPs (similar to 215 nm) with different surface charge via SPG membrane emulsification technique and deposition method. They possess uniform size as well as identical other physicochemical properties, minimizing any differences between the NPs except for surface charge. Moreover, we extend our research to eight cell lines, which could help to obtain a representative conclusion. Results show that the cellular uptake rate and amount are both positively correlated with the surface charge in all cell line. Subsequent intracellular trafficking indicates that some of positively charged NPs could escape from lysosome after being internalized and exhibit perinuclear localization, whereas the negatively and neutrally charged NPs prefer to colocalize with lysosome. These results are critical in building the knowledge base required to design chitosan-based NPs to be used efficiently and specifically. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Organic ; Polymer Science |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 关键词[WOS] | MEMBRANE EMULSIFICATION TECHNIQUE ; MACROMOLECULAR THERAPEUTICS ; QUATERNIZED CHITOSAN ; GOLD NANOPARTICLES ; DELIVERY ; CARRIER ; DESIGN ; DRUG ; HYDROGELS ; MICROSPHERES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000292617700003 |
| 公开日期 | 2013-11-01 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4699]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yue, Zhan-Guo,Wei, Wei,Lv, Pi-Ping,et al. Surface Charge Affects Cellular Uptake and Intracellular Trafficking of Chitosan-Based Nanoparticles[J]. BIOMACROMOLECULES,2011,12(7):2440-2446. |
| APA | Yue, Zhan-Guo.,Wei, Wei.,Lv, Pi-Ping.,Yue, Hua.,Wang, Lian-Yan.,...&Ma, Guang-Hui.(2011).Surface Charge Affects Cellular Uptake and Intracellular Trafficking of Chitosan-Based Nanoparticles.BIOMACROMOLECULES,12(7),2440-2446. |
| MLA | Yue, Zhan-Guo,et al."Surface Charge Affects Cellular Uptake and Intracellular Trafficking of Chitosan-Based Nanoparticles".BIOMACROMOLECULES 12.7(2011):2440-2446. |
入库方式： OAI收割
来源：过程工程研究所
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