Improved stability of the carbon nanotubes-enzyme bioconjugates by biomimetic silicification
文献类型：期刊论文
| 作者 | Wang, Qi1,2; Zhou, Liya1; Jiang, Yanjun1,2; Gao, Jing1 |
| 刊名 | ENZYME AND MICROBIAL TECHNOLOGY
 |
| 出版日期 | 2011-06-10 |
| 卷号 | 49期号:1页码:11-16 |
| 关键词 | Bioconjugate Multi-walled carbon nanotubes Biomimetic silicification Papain Immobilization |
| ISSN号 | 0141-0229 |
| 通讯作者 | Gao, J |
| 英文摘要 | Nano-materials have been applied in many fields due to their excellent characteristics, such as the high surface area-to-volume ratio, excellent physicochemical properties and biological compatibility. In this study, multi-walled carbon nanotubes (MWCNTs) were utilized to prepare MWCNTs-papain bioconjugates and then realized the immobilization of papain. MWCNTs functionalized with carboxyl- and amine- groups on their surface were used as immobilization carriers. The immobilization of papain on the functionalized MWCNTs through physical absorption was examined. The conjugates were denoted as MWCNTs-papain bioconjugates. To improve the stability, the bioconjugates were further coated by silica through the biomimetic silicification process that induced by papain (denoted as silica-coated bioconjugates). The as-prepared MWCNTs-papain bioconjugates and the silica-coated bioconjugates were characterized by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy. The preliminary results showed that the bioconjugates could retain most of the initial activity of papain. Compared to free papain and MWCNTs-papain bioconjugates, the silica-coated bioconjugates exhibited significantly improved thermal, pH and recycling stability. Comparisons of the kinetic parameters between MWCNTs-papain bioconjugates and the silica-coated bioconjugates revealed that the K(m), value of the immobilized papain experienced a slight increase after silica coating, which suggested that the silica coating did not significantly hinder papain's access to substrate or release of product. (C) 2011 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | PAPAIN ; IMMOBILIZATION ; CHITOSAN ; SILICA ; COMPOSITES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000292231500003 |
| 公开日期 | 2013-11-05 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4877]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Hebei Univ Technol, Sch Chem Engn, Tianjin 300130, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Qi,Zhou, Liya,Jiang, Yanjun,et al. Improved stability of the carbon nanotubes-enzyme bioconjugates by biomimetic silicification[J]. ENZYME AND MICROBIAL TECHNOLOGY,2011,49(1):11-16. |
| APA | Wang, Qi,Zhou, Liya,Jiang, Yanjun,&Gao, Jing.(2011).Improved stability of the carbon nanotubes-enzyme bioconjugates by biomimetic silicification.ENZYME AND MICROBIAL TECHNOLOGY,49(1),11-16. |
| MLA | Wang, Qi,et al."Improved stability of the carbon nanotubes-enzyme bioconjugates by biomimetic silicification".ENZYME AND MICROBIAL TECHNOLOGY 49.1(2011):11-16. |
入库方式： OAI收割
来源：过程工程研究所
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