One-step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on cross-linked 12% agarose gel
文献类型：期刊论文
| 作者 | Liu, Dan2; Ma, Yan3; Wang, Ye4; Su, Zhiguo1; Gu, Ming1; Janson, Jan-Christer5 |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2011-05-01 |
| 卷号 | 34期号:9页码:995-998 |
| 关键词 | Adsorption chromatography Cross-linked agarose gel Hydrolysable tannin |
| ISSN号 | 1615-9306 |
| 通讯作者 | Gu, M |
| 英文摘要 | The hydrolysable tannins corilagin and geraniin, the major active components of the traditional Chinese medicine Geranium wilfordii Maxim, have been separated and purified from crude extracts in one step by adsorption chromatography on cross-linked 12% agarose gel (Superose 12 10/300 GL). The separation was achieved by gradient elution using mobile phase A composed of 5% ethanol and 5% acetic acid and mobile phase B composed of 30% ethanol and 30% acetic acid. The gradients were composed as follows: 0-240 mL, 0-25% B; 240-480 mL, 25-40% B; after 480 mL, 100% B. The purities of the collected corilagin and geraniin were 92.4 and 87.2%, and the corresponding yields were 88.0 and 76.8%, respectively. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | CENTRIFUGAL PARTITION CHROMATOGRAPHY ; COUNTER-CURRENT CHROMATOGRAPHY ; CORILAGIN ; MEDIA ; ACID ; L. ; POLYPHENOLS ; ANTIOXIDANT ; CHEMISTRY ; GALLATE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000290634600004 |
| 公开日期 | 2013-11-05 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4883]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Dalian Univ Technol, Sch Environm & Biol Sci & Technol, Dalian, Peoples R China 3.Jiangsu Membrane Separat Co Ltd, Dalian, Peoples R China 4.Nanjing Agr Univ, Nanjing, Peoples R China 5.Uppsala Univ, Uppsala, Sweden |
| 推荐引用方式 GB/T 7714 | Liu, Dan,Ma, Yan,Wang, Ye,et al. One-step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on cross-linked 12% agarose gel[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(9):995-998. |
| APA | Liu, Dan,Ma, Yan,Wang, Ye,Su, Zhiguo,Gu, Ming,&Janson, Jan-Christer.(2011).One-step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on cross-linked 12% agarose gel.JOURNAL OF SEPARATION SCIENCE,34(9),995-998. |
| MLA | Liu, Dan,et al."One-step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on cross-linked 12% agarose gel".JOURNAL OF SEPARATION SCIENCE 34.9(2011):995-998. |
入库方式： OAI收割
来源：过程工程研究所
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