Preparation of Ca-Alginate Microparticles and Its Application for Phenylketonuria Oral Therapy
文献类型：期刊论文
| 作者 | Zhang, Yueling1; Jia, Xingyuan2,3,4; Wang, Lianyan1; Liu, Jingzhong4; Ma, Guanghui1 |
| 刊名 | INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
 |
| 出版日期 | 2011-04-06 |
| 卷号 | 50期号:7页码:4106-4112 |
| 关键词 | PHENYLALANINE AMMONIA-LYASE ELECTROSTATIC DROPLET GENERATION DRUG-DELIVERY CHITOSAN MICROSPHERES METABOLISM ADULTS ACID |
| ISSN号 | 0888-5885 |
| 通讯作者 | Wang, LY |
| 英文摘要 | Lactococcus lactis-expressing phenylalanine ammonia-lyase (LLEP) has been used to treat one of the classic genetic diseases, phenylketonuria (PKU). However, the action of stomach fluid and short residence time of LLEP at the site of absorption become the "neck" for the oral administration of LLEP. To solve these problems, pH-sensitive Ca-alginate microparticles designed as an oral administration carrier were prepared by a spray-solidification method in this study. The spray conditions influenced the size of the Ca-alginate micropartides; thus, conditions were optimized to obtain microparticles with smaller particle size for oral administration to mice. Subsequently, LLEP was encapsulated into Ca-alginate microparticles and the activity retention of LLEP released from the micropartides was examined after the microparticles passed through simulated gastric fluid. The results showed that LLEP could be well protected against simulated gastric fluid and that the final activity retention was up to 92.9%. The effects of alginate concentration on the release profile in vitro and the encapsulation efficiency (EE) were studied, and the results revealed that the microparticles possessed the highest EE and a reasonable release rate when the alginate concentration was 1.0 wt %. This alginate concentration, combined with optimized spray conditions, was used to prepare LLEP-encapsulated microparticles, and they were administered orally to mice with phenylketonuria (PKU). Compared with the groups given blank microparticles and non-encapsulated LLEP, the increase of the blood phenylalanine (Phe) level was significantly slowed after a 7 day treatment with LLEP-encapsulated microparticles. Consequently, the Ca-alginate microparticle developed by the spray-solidification method is a promising carrier of LLEP for oral administration. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Chemical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | PHENYLALANINE AMMONIA-LYASE ; ELECTROSTATIC DROPLET GENERATION ; DRUG-DELIVERY ; CHITOSAN ; MICROSPHERES ; METABOLISM ; ADULTS ; ACID |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000288833700050 |
| 公开日期 | 2013-11-06 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5050]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Med Sci, Inst Basic Med Sci, Beijing 100005, Peoples R China 3.Peking Union Med Coll, Beijing 100005, Peoples R China 4.Capital Med Univ, Beijing Chaoyang Hosp, Beijing 100020, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yueling,Jia, Xingyuan,Wang, Lianyan,et al. Preparation of Ca-Alginate Microparticles and Its Application for Phenylketonuria Oral Therapy[J]. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,2011,50(7):4106-4112. |
| APA | Zhang, Yueling,Jia, Xingyuan,Wang, Lianyan,Liu, Jingzhong,&Ma, Guanghui.(2011).Preparation of Ca-Alginate Microparticles and Its Application for Phenylketonuria Oral Therapy.INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,50(7),4106-4112. |
| MLA | Zhang, Yueling,et al."Preparation of Ca-Alginate Microparticles and Its Application for Phenylketonuria Oral Therapy".INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH 50.7(2011):4106-4112. |
入库方式： OAI收割
来源：过程工程研究所
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