Kinetic and stoichiometric analysis of the modification process for N-terminal PEGylation of staphylokinase
文献类型：期刊论文
| 作者 | Wang, Jun1,2; Hu, Tao1; Liu, Yongdong1; Zhang, Guifeng1; Ma, Guanghui1; Su, Zhiguo1 |
| 刊名 | ANALYTICAL BIOCHEMISTRY
 |
| 出版日期 | 2011-05-01 |
| 卷号 | 412期号:1页码:114-116 |
| 关键词 | RECOMBINANT STAPHYLOKINASE POLY(ETHYLENE GLYCOL) PROTEINS PLASMINOGEN |
| ISSN号 | 0003-2697 |
| 通讯作者 | Hu, T |
| 英文摘要 | Staphylokinase (SAK) is a therapeutic protein with promise for thrombolytic therapy of acute myocardial infarction. In this study, polyethylene glycol (PEG) aldehyde was used for N-terminal PEGylation of SAK to improve the pharmacological profiles of SAK. Due to the presence of the competitive PEGylation between the N terminus and the Lys residues, kinetic and stoichiometric analysis was carried out to investigate the process for the N-terminal PEGylation of SAK. To achieve this objective, size exclusion chromatography and tryptic peptide mapping were used to measure the PEGylation extent of SAIL molecule and its specific amino acid residues, respectively. (C) 2010 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | RECOMBINANT STAPHYLOKINASE ; POLY(ETHYLENE GLYCOL) ; PROTEINS ; PLASMINOGEN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000288589700018 |
| 公开日期 | 2013-11-06 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5068]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jun,Hu, Tao,Liu, Yongdong,et al. Kinetic and stoichiometric analysis of the modification process for N-terminal PEGylation of staphylokinase[J]. ANALYTICAL BIOCHEMISTRY,2011,412(1):114-116. |
| APA | Wang, Jun,Hu, Tao,Liu, Yongdong,Zhang, Guifeng,Ma, Guanghui,&Su, Zhiguo.(2011).Kinetic and stoichiometric analysis of the modification process for N-terminal PEGylation of staphylokinase.ANALYTICAL BIOCHEMISTRY,412(1),114-116. |
| MLA | Wang, Jun,et al."Kinetic and stoichiometric analysis of the modification process for N-terminal PEGylation of staphylokinase".ANALYTICAL BIOCHEMISTRY 412.1(2011):114-116. |
入库方式： OAI收割
来源：过程工程研究所
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