Enzyme adsorption and recycling during hydrolysis of wheat straw lignocellulose
文献类型：期刊论文
| 作者 | Qi, Benkun; Chen, Xiangrong; Su, Yi; Wan, Yinhua |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2011-02-01 |
| 卷号 | 102期号:3页码:2881-2889 |
| 关键词 | Cellulase Adsorption Ultrafiltration Enzyme recycling Enzyme recovery |
| ISSN号 | 0960-8524 |
| 通讯作者 | Wan, YH |
| 英文摘要 | The objective of this research was to investigate cellulase adsorption and recycling during enzymatic hydrolysis of two differently pretreated wheat straws (WS). Dilute acid treated WS showed lower hydrolysis yield of polysaccharides fraction and adsorbed more cellulase with hydrolyzed residue than dilute alkali treated sample. Four methods capable of recovering and recycling the enzyme bound to the residual substrate and the enzyme free in solution were used for three consecutive rounds of hydrolysis to compare their recycling efficiencies. Compared to the absorption recycling method, ultrafiltration recycling method possessed the capacity to retain p-glucosidase, thereby avoiding the supplementation of fresh p-glucosidase in subsequent rounds of hydrolysis. It was found that whatever recycling method was used, better recycling results were obtained for dilute alkali treated substrate than for dilute acid treated substrate. These results suggested that the great difference in the lignin content between acid treated WS and alkali treated WS would significantly affect enzymatic hydrolysis, cellulase adsorption and cellulase recycling efficiencies. (C) 2010 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | CELLULASE ADSORPTION ; CORN STOVER ; RECOVERY ; SOFTWOOD ; LIGNIN ; OPTIMIZATION ; COMPLEX ; ETHANOL ; BIOMASS ; SACCHARIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000286904500104 |
| 公开日期 | 2013-11-08 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5262]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Benkun,Chen, Xiangrong,Su, Yi,et al. Enzyme adsorption and recycling during hydrolysis of wheat straw lignocellulose[J]. BIORESOURCE TECHNOLOGY,2011,102(3):2881-2889. |
| APA | Qi, Benkun,Chen, Xiangrong,Su, Yi,&Wan, Yinhua.(2011).Enzyme adsorption and recycling during hydrolysis of wheat straw lignocellulose.BIORESOURCE TECHNOLOGY,102(3),2881-2889. |
| MLA | Qi, Benkun,et al."Enzyme adsorption and recycling during hydrolysis of wheat straw lignocellulose".BIORESOURCE TECHNOLOGY 102.3(2011):2881-2889. |
入库方式： OAI收割
来源：过程工程研究所
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