Salt Induced Irreversible Protein Adsorption with Extremely High Loadings on Electrospun Nanofibers
文献类型：期刊论文
| 作者 | Liu, Chun-Xia1,2; Zhang, Song-Ping1; Su, Zhi-Guo1; Wang, Ping3,4 |
| 刊名 | LANGMUIR
 |
| 出版日期 | 2011-01-18 |
| 卷号 | 27期号:2页码:760-765 |
| 关键词 | POLYMER NANOFIBRES METAL-IONS BEHAVIOR IMMOBILIZATION BIOCATALYSTS DESORPTION STABILITY MEMBRANES BIOMASS |
| ISSN号 | 0743-7463 |
| 通讯作者 | Su, ZG |
| 英文摘要 | LiCl is a kosmotrope that generally promotes protein salvation in aqueous solutions. Herein we report that LiCl embedded in electrospun polymeric nanofibers interestingly induced an abnormal protein adsorption and substantially augmented the adsorption capacity of the fibers. As a result, equilibrium protein loadings reached over 64% (w/w) of the dry mass of fibers, 9-fold higher than that observed in the absence of the salt. The adsorption appeared to be irreversible such that little protein loss was observed even after washing the fibers vigorously with fresh buffer solutions. We further examined the application of such intensified protein adsorption for enzyme immobilization. Proteins including bovine serum albumin (BSA) and prolamine were first adsorbed, followed by covalent attachment of an outer layer of an enzyme, alpha-chymotrypsin. Such a multilayer-structured nanofibrous enzyme exhibited extremely high stability with no obvious activity loss even after being incubated for 8 months at 4 degrees C in aqueous buffer solution. The LiCl induced irreversible protein adsorption, which has been largely ignored in previous studies with electrospun materials, rendering an interesting scenario of interfacial protein-material interactions. It also reveals a new mechanism in controlling and fabricating molecular interactions at interfaces for development of a broad range of biomaterials. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| 类目[WOS] | Chemistry, Multidisciplinary ; Chemistry, Physical ; Materials Science, Multidisciplinary |
| 研究领域[WOS] | Chemistry ; Materials Science |
| 关键词[WOS] | POLYMER NANOFIBRES ; METAL-IONS ; BEHAVIOR ; IMMOBILIZATION ; BIOCATALYSTS ; DESORPTION ; STABILITY ; MEMBRANES ; BIOMASS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000285990500038 |
| 公开日期 | 2013-11-08 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5376]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA 4.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA |
| 推荐引用方式 GB/T 7714 | Liu, Chun-Xia,Zhang, Song-Ping,Su, Zhi-Guo,et al. Salt Induced Irreversible Protein Adsorption with Extremely High Loadings on Electrospun Nanofibers[J]. LANGMUIR,2011,27(2):760-765. |
| APA | Liu, Chun-Xia,Zhang, Song-Ping,Su, Zhi-Guo,&Wang, Ping.(2011).Salt Induced Irreversible Protein Adsorption with Extremely High Loadings on Electrospun Nanofibers.LANGMUIR,27(2),760-765. |
| MLA | Liu, Chun-Xia,et al."Salt Induced Irreversible Protein Adsorption with Extremely High Loadings on Electrospun Nanofibers".LANGMUIR 27.2(2011):760-765. |
入库方式： OAI收割
来源：过程工程研究所
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