Simultaneous production of 1,3-dihydroxyacetone and xylitol from glycerol and xylose using a nanoparticle-supported multi-enzyme system with in situ cofactor regeneration
文献类型：期刊论文
| 作者 | Zhang, Ying1,2; Gao, Fei1,2; Zhang, Song-Ping1; Su, Zhi-Guo1; Ma, Guang-Hui1; Wang, Ping3,4 |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 102期号:2页码:1837-1843 |
| 关键词 | Enzymatic biocatalysis Cofactor regeneration Glycerol dehydrogenase Xylose reductase Biosynthesis |
| ISSN号 | 0960-8524 |
| 通讯作者 | Ma, GH |
| 英文摘要 | Cofactor-dependent biotransformations often require consumption of a secondary substrate for cofactor regeneration. Alternatively, two synthetic reactions may be coupled together through cofactor regeneration cycles. Simultaneous production of value-added products from glycerol and xylose was realized in this work through an enzymatic NAD(H) regeneration cycle involving two enzymes. Glycerol dehydrogenase (GDH) catalyzed the production of 1,3-dihydroxyacetone (DHA) from glycerol, while xylose reductase (XR) enabled the reduction of xylose to xylitol using the protons released from glycerol. Both enzymes were immobilized with P(MMA-EDMA-MAA) nanoparticles. Interestingly, the immobilized multi-enzyme system showed much improved productivity and stability as compared to native enzymes, such that the total turnover number (TTN) reached 82 for cofactor regeneration while the yield reached 160 g/g-immobilized GDH for DHA production. (C) 2010 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | BIOCATALYTIC ACTIVITY ; CARBON NANOTUBES ; MEMBRANE REACTOR ; SINGLE-ENZYME ; DEHYDROGENASE ; NANOFIBERS ; STABILITY ; ACID |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000286782700196 |
| 公开日期 | 2013-11-08 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5383]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA 4.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA |
| 推荐引用方式 GB/T 7714 | Zhang, Ying,Gao, Fei,Zhang, Song-Ping,et al. Simultaneous production of 1,3-dihydroxyacetone and xylitol from glycerol and xylose using a nanoparticle-supported multi-enzyme system with in situ cofactor regeneration[J]. BIORESOURCE TECHNOLOGY,2011,102(2):1837-1843. |
| APA | Zhang, Ying,Gao, Fei,Zhang, Song-Ping,Su, Zhi-Guo,Ma, Guang-Hui,&Wang, Ping.(2011).Simultaneous production of 1,3-dihydroxyacetone and xylitol from glycerol and xylose using a nanoparticle-supported multi-enzyme system with in situ cofactor regeneration.BIORESOURCE TECHNOLOGY,102(2),1837-1843. |
| MLA | Zhang, Ying,et al."Simultaneous production of 1,3-dihydroxyacetone and xylitol from glycerol and xylose using a nanoparticle-supported multi-enzyme system with in situ cofactor regeneration".BIORESOURCE TECHNOLOGY 102.2(2011):1837-1843. |
入库方式： OAI收割
来源：过程工程研究所
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