Bioinspired preparation of polydopamine microcapsule for multienzyme system construction
文献类型：期刊论文
| 作者 | Zhang, Lei1,2; Shi, Jiafu1,2; Jiang, Zhongyi1,2; Jiang, Yanjun3; Qiao, Shizhang4; Li, Jian1; Wang, Rui1; Meng, Ruijie1; Zhu, Yuanyuan1; Zheng, Yang1 |
| 刊名 | GREEN CHEMISTRY
 |
| 出版日期 | 2011 |
| 卷号 | 13期号:2页码:300-306 |
| 关键词 | SURFACE-CHEMISTRY POLYMER CAPSULES POLYELECTROLYTE ENZYMES MICROPARTICLES ENCAPSULATION TEMPLATES PARTICLES DOPAMINE COATINGS |
| ISSN号 | 1463-9262 |
| 通讯作者 | Zhang, L |
| 英文摘要 | Inspired by the structural organization of mitochondria and the bioadhesive principle, a simple and versatile approach to construct a multienzyme system is developed. More specifically, the multienzyme system is composed of a polydopamine (PDA) microcapsule scaffold and three spatially separated enzymes. The PDA microcapsules are prepared through the rapid, spontaneous self-polymerization of dopamine on the surface of CaCO(3) microparticle template, followed by dissolution of the template using EDTA. The wall thickness of the microcapsules can be tuned by the dopamine concentration in an aqueous solution. The three enzymes are respectively immobilized through physical encapsulation in the lumen, in situ entrapment within the wall and chemical attachment on the out surface under extremely mild conditions. As an example, a multienzyme system, containing alpha-amylase, beta-amylase and glucosidase, was constructed to convert starch into isomaltooligosaccharide, and the multienzyme system displays higher catalytic activity and enhanced operational stability. The method developed in this study will establish a powerful platform for the facile construction of multienzyme cascade systems. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | SURFACE-CHEMISTRY ; POLYMER CAPSULES ; POLYELECTROLYTE ; ENZYMES ; MICROPARTICLES ; ENCAPSULATION ; TEMPLATES ; PARTICLES ; DOPAMINE ; COATINGS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000287092500010 |
| 公开日期 | 2013-11-08 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5388]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Tianjin Univ, Sch Chem Engn & Technol, Minist Educ, Key Lab Green Technol, Tianjin 300072, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Hebei Univ Technol, Sch Chem Engn, Dept Bioengn, Tianjin 300130, Peoples R China 4.Univ Queensland, Australian Inst Bioengn & Nanotechnol, ARC Ctr Excellence Funct Nanomat, Brisbane, Qld 4072, Australia |
| 推荐引用方式 GB/T 7714 | Zhang, Lei,Shi, Jiafu,Jiang, Zhongyi,et al. Bioinspired preparation of polydopamine microcapsule for multienzyme system construction[J]. GREEN CHEMISTRY,2011,13(2):300-306. |
| APA | Zhang, Lei.,Shi, Jiafu.,Jiang, Zhongyi.,Jiang, Yanjun.,Qiao, Shizhang.,...&Zheng, Yang.(2011).Bioinspired preparation of polydopamine microcapsule for multienzyme system construction.GREEN CHEMISTRY,13(2),300-306. |
| MLA | Zhang, Lei,et al."Bioinspired preparation of polydopamine microcapsule for multienzyme system construction".GREEN CHEMISTRY 13.2(2011):300-306. |
入库方式： OAI收割
来源：过程工程研究所
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