Melatonin improves the survival of cryopreserved callus of Rhodiola crenulata
文献类型：期刊论文
| 作者 | Zhao, Yan1,2; Qi, Li-Wang3; Wang, Wei-Ming4; Saxena, Praveen K.5; Liu, Chun-Zhao1,2 |
| 刊名 | JOURNAL OF PINEAL RESEARCH
 |
| 出版日期 | 2011 |
| 卷号 | 50期号:1页码:83-88 |
| 关键词 | antioxidative enzymes cryopreservation malondialdehyde melatonin Rhodiola crenulata |
| ISSN号 | 0742-3098 |
| 通讯作者 | Liu, CZ |
| 英文摘要 | An important aspect of the function of melatonin seems to be the mediation of stress caused by environmental and chemical factors. In the cryopreservation process, environmental changes including osmotic injury, desiccation, and low temperature can impose a series of stresses on plants. In this study, we evaluated the role of melatonin in stress protection during the process of cryopreservation using callus of an endangered plant species Rhodiola crenulata. The survival rate of the cryopreserved callus significantly increased when the callus was pretreated for 5 days with 0.1 mu m melatonin prior to freezing in liquid nitrogen. Analysis of antioxidative activity following the pretreatment of callus with 0.1 mu m melatonin showed a significant reduction in malondialdehyde production during various steps of cryopreservation. Enhanced peroxidase and catalase activity was observed in the callus after pretreatment with 0.1 mu m melatonin compared to the control. These observations provide new evidence of the antioxidant/anti-stress function of melatonin, and it is the first report of its potential application in the preservation of elite endangered germplasm through the process of cryopreservation. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Endocrinology & Metabolism ; Neurosciences ; Physiology |
| 研究领域[WOS] | Endocrinology & Metabolism ; Neurosciences & Neurology ; Physiology |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; SHOOT-TIPS ; SUPEROXIDE-DISMUTASE ; ANTIOXIDANT ENZYMES ; PEROXIDASE-ACTIVITY ; OXIDATIVE STRESS ; EUGLENA-GRACILIS ; VITRIFICATION ; PLANTS ; CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000285154400011 |
| 公开日期 | 2013-11-11 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5400]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100190, Peoples R China 3.Chinese Acad Forestry, Res Inst Forestry, Beijing, Peoples R China 4.Phyton Biotech Inc, E Windsor, NJ USA 5.Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada |
| 推荐引用方式 GB/T 7714 | Zhao, Yan,Qi, Li-Wang,Wang, Wei-Ming,et al. Melatonin improves the survival of cryopreserved callus of Rhodiola crenulata[J]. JOURNAL OF PINEAL RESEARCH,2011,50(1):83-88. |
| APA | Zhao, Yan,Qi, Li-Wang,Wang, Wei-Ming,Saxena, Praveen K.,&Liu, Chun-Zhao.(2011).Melatonin improves the survival of cryopreserved callus of Rhodiola crenulata.JOURNAL OF PINEAL RESEARCH,50(1),83-88. |
| MLA | Zhao, Yan,et al."Melatonin improves the survival of cryopreserved callus of Rhodiola crenulata".JOURNAL OF PINEAL RESEARCH 50.1(2011):83-88. |
入库方式： OAI收割
来源：过程工程研究所
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