Particle size affects the cellular response in macrophages
文献类型：期刊论文
| 作者 | Yue, Hua1,2; Wei, Wei1,2; Yue, Zhanguo1,2; Lv, Piping1,2; Wang, Lianyan1; Ma, Guanghui1; Su, Zhiguo1 |
| 刊名 | EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
 |
| 出版日期 | 2010-12-23 |
| 卷号 | 41期号:5页码:650-657 |
| 关键词 | Biomaterials Cellular response Macrophage Particle design Size |
| ISSN号 | 0928-0987 |
| 通讯作者 | Ma, GH |
| 英文摘要 | A deeper understanding of how the physical properties of particles regulate specific biological responses is becoming a crucial requirement for their successful biomedical application. To provide insights on their design and application, J774A.1 cells are exposed to particles with different diameters (430 nm, 1.9 mu m and 4.8 mu m), and the size effects on a series of cellular responses in macrophages are evaluated. Cellular uptake study demonstrates that nanosized particles accumulate in the cells at a faster rate, and with a higher surface area. Once the data are converted into the expression of particle volume, the maximum value is found with 1.9 mu m particles instead of nanoparticles. Moreover, the uptake intermediates are also trapped, and the steps of particle internalization include filopodia sensing, skeleton rearrangement, and morphology change. Subsequent cellular trafficking reveals that only nanosized particles transport via lysosomal pathway, which is consistent with their uptake mechanisms. Furthermore, nanosized particles prefer to promote the secretion of Th1-specific molecule signals (e.g. IFN, IL-12) rather than immune suppressors. All these results, along with a couple of surprises, are discussed in the view of clinical practice. They are expected, in principle, to establish the basis of new design concepts for particle-based biomedical applications. (C) 2010 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Pharmacology & Pharmacy |
| 研究领域[WOS] | Pharmacology & Pharmacy |
| 关键词[WOS] | DRUG-DELIVERY ; GOLD NANOPARTICLES ; SURFACE-PROPERTIES ; VACCINE ADJUVANTS ; DENDRITIC CELLS ; PATHWAYS ; DESIGN ; INTERNALIZATION ; MICROSPHERES ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000285451300011 |
| 公开日期 | 2013-11-11 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5406]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yue, Hua,Wei, Wei,Yue, Zhanguo,et al. Particle size affects the cellular response in macrophages[J]. EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES,2010,41(5):650-657. |
| APA | Yue, Hua.,Wei, Wei.,Yue, Zhanguo.,Lv, Piping.,Wang, Lianyan.,...&Su, Zhiguo.(2010).Particle size affects the cellular response in macrophages.EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES,41(5),650-657. |
| MLA | Yue, Hua,et al."Particle size affects the cellular response in macrophages".EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES 41.5(2010):650-657. |
入库方式： OAI收割
来源：过程工程研究所
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