An automatic system for multidimensional integrated protein chromatography
文献类型：期刊论文
| 作者 | Kong, Yingjun1,2; Li, Xiunan1; Bai, Gaoying3; Ma, Guanghui1; Su, Zhiguo1 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2010-10-29 |
| 卷号 | 1217期号:44页码:6898-6904 |
| 关键词 | Multidimensional Biorefinery Chromatography Protein purification |
| ISSN号 | 0021-9673 |
| 通讯作者 | Li, XN |
| 英文摘要 | An automatic system for multidimensional integrated protein chromatography was designed for simultaneous separation of multiple proteins from complex mixtures, such as human plasma and tissue lysates. This computer-controlled system integrates several chromatographic columns that work independently or cooperatively with one another to achieve efficient high throughputs. The pipelines can be automatically switched either to another column or to a collection container for each UV-detected elution fraction. Environmental contamination is avoided due to the closed fluid paths and elimination of manual column change. This novel system was successfully used for simultaneous preparation of five proteins from the precipitate of human plasma fraction IV (fraction IV). The system involved gel filtration, ion exchange, hydrophobic interaction, and heparin affinity chromatography. Human serum albumin (HSA), transferrin (TO, antithrombin-III (AT-III), alpha 1-antitrypsin (alpha 1-AT), and haptoglobin (Hp) were purified within 3 h. The following recovery and purity were achieved: 95% (RSD, 2.8%) and 95% for HSA, 80% (RSD, 2.0%) and 99% for Tf, 70% (RSD, 2.1%) and 99% for AT-III, 65% (RSD, 2.0%) and 94% for alpha 1-AT, and 50% (RSD, 1.0%) and 90% for Hp. The results demonstrate that this novel multidimensional integrated chromatography system is capable of simultaneously separating multiple protein products from the same raw material with high yield and purity and it has the potential for a wide range of multi-step chromatography separation processes. (c) 2010 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | ANTIBODY AFFINITY-CHROMATOGRAPHY ; LIQUID-CHROMATOGRAPHY ; MILLIGRAM QUANTITIES ; ANTITHROMBIN-III ; HAPTOGLOBIN 1-1 ; PURIFICATION ; IDENTIFICATION ; TRANSFERRIN ; STRATEGIES ; SERUM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000283804800012 |
| 公开日期 | 2013-11-12 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5690]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Kong, Yingjun,Li, Xiunan,Bai, Gaoying,et al. An automatic system for multidimensional integrated protein chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2010,1217(44):6898-6904. |
| APA | Kong, Yingjun,Li, Xiunan,Bai, Gaoying,Ma, Guanghui,&Su, Zhiguo.(2010).An automatic system for multidimensional integrated protein chromatography.JOURNAL OF CHROMATOGRAPHY A,1217(44),6898-6904. |
| MLA | Kong, Yingjun,et al."An automatic system for multidimensional integrated protein chromatography".JOURNAL OF CHROMATOGRAPHY A 1217.44(2010):6898-6904. |
入库方式： OAI收割
来源：过程工程研究所
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