Affinity adsorption of albumin on Cibacron Blue F3GA-coupled non-porous micrometer-sized magnetic polymer microspheres
文献类型：期刊论文
| 作者 | Ma, ZY; Guan, YP; Lu, HZ |
| 刊名 | REACTIVE & FUNCTIONAL POLYMERS
 |
| 出版日期 | 2006-06-01 |
| 卷号 | 66期号:6页码:618-624 |
| 关键词 | Cibacron Blue F3GA PSt-DVB-GMA affinity adsorption magnetic microspheres bovine serum albumin |
| ISSN号 | 1381-5148 |
| 其他题名 | React. Funct. Polym. |
| 中文摘要 | Non-porous micrometer-sized magnetic polymer microspheres were prepared for affinity protein adsorption utilizing Cibacron Blue F3GA as a dye ligand. Magnetic poly (styrene-divinylbenzene-glycidyl methacrylate) (PSt-DVB-GMA) microspheres were prepared by a modified suspension polymerization method. The magnetic microspheres were modified with ethylenediamine by ring-opening reaction of epoxy groups to produce surface functional amino groups. Then, Cibacron Blue F3GA was covalently immobilized onto the above magnetic microspheres for bovine serum albumin (BSA) adsorption from aqueous solution. Scanning electron microscopy showed that the magnetic microspheres had an average size of 6 mu m and magnetic measurement revealed they were superparamagnetic with saturation magnetization of 7.3 emu/g. Albumin adsorption showed that the dye ligand attached magnetic microspheres had high adsorption capacity of BSA (80.2mg/g) and low non-specific adsorption (< 10mg/g). The adsorption conditions, such as pH, ionic strength and initial concentration of BSA were investigated. Desorption of BSA was obtained using 1 M NaSCN solution. (c) 2005 Elsevier B.V. All rights reserved. |
| 英文摘要 | Non-porous micrometer-sized magnetic polymer microspheres were prepared for affinity protein adsorption utilizing Cibacron Blue F3GA as a dye ligand. Magnetic poly (styrene-divinylbenzene-glycidyl methacrylate) (PSt-DVB-GMA) microspheres were prepared by a modified suspension polymerization method. The magnetic microspheres were modified with ethylenediamine by ring-opening reaction of epoxy groups to produce surface functional amino groups. Then, Cibacron Blue F3GA was covalently immobilized onto the above magnetic microspheres for bovine serum albumin (BSA) adsorption from aqueous solution. Scanning electron microscopy showed that the magnetic microspheres had an average size of 6 mu m and magnetic measurement revealed they were superparamagnetic with saturation magnetization of 7.3 emu/g. Albumin adsorption showed that the dye ligand attached magnetic microspheres had high adsorption capacity of BSA (80.2mg/g) and low non-specific adsorption (< 10mg/g). The adsorption conditions, such as pH, ionic strength and initial concentration of BSA were investigated. Desorption of BSA was obtained using 1 M NaSCN solution. (c) 2005 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| 类目[WOS] | Chemistry, Applied ; Engineering, Chemical ; Polymer Science |
| 研究领域[WOS] | Chemistry ; Engineering ; Polymer Science |
| 关键词[WOS] | PROTEIN ADSORPTION ; CHROMATOGRAPHY ; PURIFICATION ; MICROBEADS ; ANTIBODIES ; SEPARATION ; PARTICLES ; SUPPORTS ; STYRENE ; PLASMA |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000238443100005 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3924]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, ZY,Guan, YP,Lu, HZ. Affinity adsorption of albumin on Cibacron Blue F3GA-coupled non-porous micrometer-sized magnetic polymer microspheres[J]. REACTIVE & FUNCTIONAL POLYMERS,2006,66(6):618-624. |
| APA | Ma, ZY,Guan, YP,&Lu, HZ.(2006).Affinity adsorption of albumin on Cibacron Blue F3GA-coupled non-porous micrometer-sized magnetic polymer microspheres.REACTIVE & FUNCTIONAL POLYMERS,66(6),618-624. |
| MLA | Ma, ZY,et al."Affinity adsorption of albumin on Cibacron Blue F3GA-coupled non-porous micrometer-sized magnetic polymer microspheres".REACTIVE & FUNCTIONAL POLYMERS 66.6(2006):618-624. |
入库方式： OAI收割
来源：过程工程研究所
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