中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Caffeic acid derivatives production by hairy root cultures of Echinacea purpurea

文献类型:期刊论文

作者Liu, Chun-Zhao; Abbasi, Bilal H.; Gao, Min; Murch, Susan J.; Saxena, Praveen K.
刊名JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
出版日期2006-11-01
卷号54期号:22页码:8456-8460
关键词Echinacea purpurea Agrobacterium rhizogenes hairy roots caffeic acid derivatives cichoric acid HPLC
ISSN号0021-8561
其他题名J. Agric. Food Chem.
中文摘要Inoculation of leaf explants of Echinacea purpurea( Moench) with Agrobacterium rhizogenes induced hairy roots with the capacity to produce biologically active caffeic acid derivatives ( CADs), especially cichoric acid. The kinetics of growth, the uptake of macronutrients, and the accumulation of CADs were investigated in heterotrophically cultured hairy roots for a 50 day period. A maximum of 12.2 g L(-1) dry biomass was achieved in MS nutrients supplemented with 30 g L(-1) sucrose on day 40. The mathematical relationship between hairy root growth and conductivity was established during the exponential phase in Erlenmeyer flasks. HPLC analyses of methanolic ( 0.1% phosphoric acid; 70: 30, v/v) extracts from hairy roots revealed the presence of important CADs: cichoric acid ( 19.21 mg g(-1) dry biomass), caftaric acid ( 3.56 mg g(-1) dry biomass), and chlorogenic acid ( 0.93 mg g(-1) dry biomass). These results demonstrate that biotechnological production of CADs in hairy roots of E. purpurea is possible. Furthermore, these hairy root cultures offer, for the very first time, an excellent biological model to study the biosynthetic pathway of medicinally important CADs.
英文摘要Inoculation of leaf explants of Echinacea purpurea( Moench) with Agrobacterium rhizogenes induced hairy roots with the capacity to produce biologically active caffeic acid derivatives ( CADs), especially cichoric acid. The kinetics of growth, the uptake of macronutrients, and the accumulation of CADs were investigated in heterotrophically cultured hairy roots for a 50 day period. A maximum of 12.2 g L(-1) dry biomass was achieved in MS nutrients supplemented with 30 g L(-1) sucrose on day 40. The mathematical relationship between hairy root growth and conductivity was established during the exponential phase in Erlenmeyer flasks. HPLC analyses of methanolic ( 0.1% phosphoric acid; 70: 30, v/v) extracts from hairy roots revealed the presence of important CADs: cichoric acid ( 19.21 mg g(-1) dry biomass), caftaric acid ( 3.56 mg g(-1) dry biomass), and chlorogenic acid ( 0.93 mg g(-1) dry biomass). These results demonstrate that biotechnological production of CADs in hairy roots of E. purpurea is possible. Furthermore, these hairy root cultures offer, for the very first time, an excellent biological model to study the biosynthetic pathway of medicinally important CADs.
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
研究领域[WOS]Agriculture ; Chemistry ; Food Science & Technology
关键词[WOS]ARTEMISIA-ANNUA L ; CATHARANTHUS-ROSEUS ; MEDIATED TRANSFORMATION ; CICHORIC ACID ; GROWTH ; BIOSYNTHESIS ; POPULATIONS ; INHIBITORS ; INTEGRASE ; ALKAMIDES
收录类别SCI
原文出处://WOS:000241519700011
语种英语
WOS记录号WOS:000241519700011
公开日期2013-10-24
版本出版稿
源URL[http://ir.ipe.ac.cn/handle/122111/3934]  
专题过程工程研究所_研究所(批量导入)
作者单位1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Phytochem Engn Grp, Beijing 100080, Peoples R China
2.Univ British Columbia, IK Barber Sch Arts & Sci, Dept Chem, Kelowna, BC V1V 1V7, Canada
3.Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada
4.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Liu, Chun-Zhao,Abbasi, Bilal H.,Gao, Min,et al. Caffeic acid derivatives production by hairy root cultures of Echinacea purpurea[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2006,54(22):8456-8460.
APA Liu, Chun-Zhao,Abbasi, Bilal H.,Gao, Min,Murch, Susan J.,&Saxena, Praveen K..(2006).Caffeic acid derivatives production by hairy root cultures of Echinacea purpurea.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,54(22),8456-8460.
MLA Liu, Chun-Zhao,et al."Caffeic acid derivatives production by hairy root cultures of Echinacea purpurea".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 54.22(2006):8456-8460.

入库方式: OAI收割

来源:过程工程研究所

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