Caffeic acid derivatives production by hairy root cultures of Echinacea purpurea
文献类型:期刊论文
作者 | Liu, Chun-Zhao; Abbasi, Bilal H.; Gao, Min; Murch, Susan J.; Saxena, Praveen K. |
刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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出版日期 | 2006-11-01 |
卷号 | 54期号:22页码:8456-8460 |
关键词 | Echinacea purpurea Agrobacterium rhizogenes hairy roots caffeic acid derivatives cichoric acid HPLC |
ISSN号 | 0021-8561 |
其他题名 | J. Agric. Food Chem. |
中文摘要 | Inoculation of leaf explants of Echinacea purpurea( Moench) with Agrobacterium rhizogenes induced hairy roots with the capacity to produce biologically active caffeic acid derivatives ( CADs), especially cichoric acid. The kinetics of growth, the uptake of macronutrients, and the accumulation of CADs were investigated in heterotrophically cultured hairy roots for a 50 day period. A maximum of 12.2 g L(-1) dry biomass was achieved in MS nutrients supplemented with 30 g L(-1) sucrose on day 40. The mathematical relationship between hairy root growth and conductivity was established during the exponential phase in Erlenmeyer flasks. HPLC analyses of methanolic ( 0.1% phosphoric acid; 70: 30, v/v) extracts from hairy roots revealed the presence of important CADs: cichoric acid ( 19.21 mg g(-1) dry biomass), caftaric acid ( 3.56 mg g(-1) dry biomass), and chlorogenic acid ( 0.93 mg g(-1) dry biomass). These results demonstrate that biotechnological production of CADs in hairy roots of E. purpurea is possible. Furthermore, these hairy root cultures offer, for the very first time, an excellent biological model to study the biosynthetic pathway of medicinally important CADs. |
英文摘要 | Inoculation of leaf explants of Echinacea purpurea( Moench) with Agrobacterium rhizogenes induced hairy roots with the capacity to produce biologically active caffeic acid derivatives ( CADs), especially cichoric acid. The kinetics of growth, the uptake of macronutrients, and the accumulation of CADs were investigated in heterotrophically cultured hairy roots for a 50 day period. A maximum of 12.2 g L(-1) dry biomass was achieved in MS nutrients supplemented with 30 g L(-1) sucrose on day 40. The mathematical relationship between hairy root growth and conductivity was established during the exponential phase in Erlenmeyer flasks. HPLC analyses of methanolic ( 0.1% phosphoric acid; 70: 30, v/v) extracts from hairy roots revealed the presence of important CADs: cichoric acid ( 19.21 mg g(-1) dry biomass), caftaric acid ( 3.56 mg g(-1) dry biomass), and chlorogenic acid ( 0.93 mg g(-1) dry biomass). These results demonstrate that biotechnological production of CADs in hairy roots of E. purpurea is possible. Furthermore, these hairy root cultures offer, for the very first time, an excellent biological model to study the biosynthetic pathway of medicinally important CADs. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
类目[WOS] | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
研究领域[WOS] | Agriculture ; Chemistry ; Food Science & Technology |
关键词[WOS] | ARTEMISIA-ANNUA L ; CATHARANTHUS-ROSEUS ; MEDIATED TRANSFORMATION ; CICHORIC ACID ; GROWTH ; BIOSYNTHESIS ; POPULATIONS ; INHIBITORS ; INTEGRASE ; ALKAMIDES |
收录类别 | SCI |
原文出处 | |
语种 | 英语 |
WOS记录号 | WOS:000241519700011 |
公开日期 | 2013-10-24 |
版本 | 出版稿 |
源URL | [http://ir.ipe.ac.cn/handle/122111/3934] ![]() |
专题 | 过程工程研究所_研究所(批量导入) |
作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Phytochem Engn Grp, Beijing 100080, Peoples R China 2.Univ British Columbia, IK Barber Sch Arts & Sci, Dept Chem, Kelowna, BC V1V 1V7, Canada 3.Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada 4.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Chun-Zhao,Abbasi, Bilal H.,Gao, Min,et al. Caffeic acid derivatives production by hairy root cultures of Echinacea purpurea[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2006,54(22):8456-8460. |
APA | Liu, Chun-Zhao,Abbasi, Bilal H.,Gao, Min,Murch, Susan J.,&Saxena, Praveen K..(2006).Caffeic acid derivatives production by hairy root cultures of Echinacea purpurea.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,54(22),8456-8460. |
MLA | Liu, Chun-Zhao,et al."Caffeic acid derivatives production by hairy root cultures of Echinacea purpurea".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 54.22(2006):8456-8460. |
入库方式: OAI收割
来源:过程工程研究所
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