Development of superparamagnetic functional carriers and application for affinity separation of subtilisin Carlsberg
文献类型：期刊论文
| 作者 | Yang, CL; Guan, YP; Xing, JM; Liu, HZ |
| 刊名 | POLYMER
 |
| 出版日期 | 2006-03-22 |
| 卷号 | 47期号:7页码:2299-2304 |
| 关键词 | magnetic functional carriers subtilisin Carlsberg affinity ligand |
| ISSN号 | 0032-3861 |
| 其他题名 | Polymer |
| 中文摘要 | Superparamagnetic functional carriers to obtain high selectivity were reported in this study. Magnetic carriers with epoxy groups were synthesized by spraying suspension polymerization (SSP). The measurement of scanning electron microscopy (SEM) and vibrating sample magnetometer (VSM) showed that the magnetic carriers have a narrow size distribution and displayed superparamagnetic characteristics. The magnetic carriers with epoxy groups were modified by various affinity ligands to magnetic functional carriers such as copper-IDA carriers, benzamidine carriers and phenylboronic acid carriers. The effects of medium pH and subtilisin Carlsberg concentration on adsorption capacity were investigated. It was found that phenylboronic acid and benzamidine immobilized magnetic carriers were effective magnetic carriers for affinity adsorption of subtilisin Carlsberg, and the maximum adsorption capacity (about 65 mg/g) was obtained at pH 9.5. The adsorbed subtilisin Carlsberg was also desorbed successfully by using dissociation agents, and the recovery of the enzyme activity was still around 85%. (c) 2006 Published by Elsevier Ltd. |
| 英文摘要 | Superparamagnetic functional carriers to obtain high selectivity were reported in this study. Magnetic carriers with epoxy groups were synthesized by spraying suspension polymerization (SSP). The measurement of scanning electron microscopy (SEM) and vibrating sample magnetometer (VSM) showed that the magnetic carriers have a narrow size distribution and displayed superparamagnetic characteristics. The magnetic carriers with epoxy groups were modified by various affinity ligands to magnetic functional carriers such as copper-IDA carriers, benzamidine carriers and phenylboronic acid carriers. The effects of medium pH and subtilisin Carlsberg concentration on adsorption capacity were investigated. It was found that phenylboronic acid and benzamidine immobilized magnetic carriers were effective magnetic carriers for affinity adsorption of subtilisin Carlsberg, and the maximum adsorption capacity (about 65 mg/g) was obtained at pH 9.5. The adsorbed subtilisin Carlsberg was also desorbed successfully by using dissociation agents, and the recovery of the enzyme activity was still around 85%. (c) 2006 Published by Elsevier Ltd. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Polymer Science |
| 研究领域[WOS] | Polymer Science |
| 关键词[WOS] | EMULSION POLYMERIZATION ; SURFACE MODIFICATION ; METHACRYLATE) MICROSPHERES ; SUSPENSION POLYMERIZATION ; ADSORPTION ; CHROMATOGRAPHY ; STYRENE ; BEADS ; PROTEINASES ; SUBTILASES |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000236629900007 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3947]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Lab Separat Sci & Engn, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 2.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, CL,Guan, YP,Xing, JM,et al. Development of superparamagnetic functional carriers and application for affinity separation of subtilisin Carlsberg[J]. POLYMER,2006,47(7):2299-2304. |
| APA | Yang, CL,Guan, YP,Xing, JM,&Liu, HZ.(2006).Development of superparamagnetic functional carriers and application for affinity separation of subtilisin Carlsberg.POLYMER,47(7),2299-2304. |
| MLA | Yang, CL,et al."Development of superparamagnetic functional carriers and application for affinity separation of subtilisin Carlsberg".POLYMER 47.7(2006):2299-2304. |
入库方式： OAI收割
来源：过程工程研究所
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