Enzyme chemiluminescence immuno assay of free hCG beta in serum based on superparamagnetic polymer microbeads
文献类型：期刊论文
| 作者 | Guo, Xiaoying; Guan, Yueping; Yang, Bin; Wang, Yongning; Lan, Hualong; Shi, Wentang; Yang, Zhenghui; Lu, Zuhong |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2006-08-01 |
| 卷号 | 7期号:8页码:274-288 |
| 关键词 | superparamagnetic polymer microbeads modified suspension polymerization enzyme chemiluminescence immunoassay free hCG beta tumor marker |
| ISSN号 | 1422-0067 |
| 其他题名 | Int. J. Mol. Sci. |
| 中文摘要 | Superparamagnetic polymers (SPMP) microbead was an excellent form for nanosized magnetic particles less than 10nm to realize their potential applications in many fields. We developed a novel modified suspension polymerization method for the production of superparamagnetic poly ( methacrylate divinylbenzene) ( PMA-DVB) microbeads, with amino groups on their surface after simple modifications. We applied these SPMP microbeads to establish a sandwich enzyme chemiluminence immuno (ECLIA) procedure of detecting the free hCG beta in serum. It was proved to a better method compared to the ELISA, since it need half of the sample volume, simpler protocols, and the time it need was shortened from 2 hours to 1 hour. The detection limit was 0.22 mIU.m(-1), one order lower than the ELISA assay. Its linear range was between 0.45 - 185.2 mIU.m(-1). And in the detections of clinical serum samples, the related coefficient between the two methods' results was 0.955. |
| 英文摘要 | Superparamagnetic polymers (SPMP) microbead was an excellent form for nanosized magnetic particles less than 10nm to realize their potential applications in many fields. We developed a novel modified suspension polymerization method for the production of superparamagnetic poly ( methacrylate divinylbenzene) ( PMA-DVB) microbeads, with amino groups on their surface after simple modifications. We applied these SPMP microbeads to establish a sandwich enzyme chemiluminence immuno (ECLIA) procedure of detecting the free hCG beta in serum. It was proved to a better method compared to the ELISA, since it need half of the sample volume, simpler protocols, and the time it need was shortened from 2 hours to 1 hour. The detection limit was 0.22 mIU.m(-1), one order lower than the ELISA assay. Its linear range was between 0.45 - 185.2 mIU.m(-1). And in the detections of clinical serum samples, the related coefficient between the two methods' results was 0.955. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | HUMAN CHORIONIC-GONADOTROPIN ; DOWN-SYNDROME ; MAGNETIC NANOPARTICLES ; FLOW IMMUNOASSAY ; CELL CARCINOMA ; 2ND TRIMESTER ; INTACT HCG ; SUBUNIT ; MICROSPHERES ; IMMUNOSENSORS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000240200800005 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3971]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.SE Univ, State Key Bioelect Lab, Nanjing 210096, Peoples R China 2.Jinchuan Grp Co Ltd, Inst Chem & Hydromet, Jinchang 737100, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 4.Chinese Acad Sci, Tech Inst Phys & Chem, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Xiaoying,Guan, Yueping,Yang, Bin,et al. Enzyme chemiluminescence immuno assay of free hCG beta in serum based on superparamagnetic polymer microbeads[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2006,7(8):274-288. |
| APA | Guo, Xiaoying.,Guan, Yueping.,Yang, Bin.,Wang, Yongning.,Lan, Hualong.,...&Lu, Zuhong.(2006).Enzyme chemiluminescence immuno assay of free hCG beta in serum based on superparamagnetic polymer microbeads.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,7(8),274-288. |
| MLA | Guo, Xiaoying,et al."Enzyme chemiluminescence immuno assay of free hCG beta in serum based on superparamagnetic polymer microbeads".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 7.8(2006):274-288. |
入库方式： OAI收割
来源：过程工程研究所
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