Ethylene gylcol diglycidyl ether as a protein cross-linker: a case study for cross-linking of hemoglobin
文献类型：期刊论文
| 作者 | Lu, ML; Xu, YH; Zheng, CY; Zhangl, GF; Su, Z |
| 刊名 | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2006-05-01 |
| 卷号 | 81期号:5页码:767-775 |
| 关键词 | ethylene glycol diglycidyl ether hemoglobin cross-linker intramolecular cross-linking intermolecular cross-linking |
| ISSN号 | 0268-2575 |
| 其他题名 | J. Chem. Technol. Biotechnol. |
| 中文摘要 | Being widely applied in the chemical industry, ethylene glycol diglycidyl ether (EGDE) was tried as a protein cross-linker for cross-linking of bovine hemoglobin (Hb). The characteristics of the reaction and the products were studied using electrophoresis and multi-angle laser light scattering coupled with HPLC. At pH 7.5, EGDE could react with the sulfhydryl group and the epsilon-amino group, but reacted preferentially with the sulfhydryl group. As the pH increased from 7.5 to 9.5, the reaction took place mainly between the amino groups and EGDE. By controlling the pH and other conditions, intra- and inter-molecularly cross-linked products with narrow size distribution were obtained. The intramolecular product was about 90% of the reaction mixture. The intermolecularly cross-linked Hb consisted of 74.7% of dimeric Hb with weight-average molecular weights (Mw) of 146 kDa, 18.9% of trimeric Hb with Mw of 228 kDa, and 6.4% of oligomer with four to six Hb units, Mw of 375 kDa. The cross-linking altered the oxygen affinity (P-50) and the Hill coefficient of Hb but they are within the range of hemoglobin-based red cell substitutes. The pI of intramolecularly and intermolecularly cross-linked Hb increased from 6.7-7.4 to 6.9-7.6 and 8.2-8.8 respectively. The study demonstrated that EGDE could be an alternative to glutaraldehyde for cross-linking of hemoglobin and other proteins on a large-scale without significantly impairing the biological activity. (c) 2006 Society of Chemical Industry. |
| 英文摘要 | Being widely applied in the chemical industry, ethylene glycol diglycidyl ether (EGDE) was tried as a protein cross-linker for cross-linking of bovine hemoglobin (Hb). The characteristics of the reaction and the products were studied using electrophoresis and multi-angle laser light scattering coupled with HPLC. At pH 7.5, EGDE could react with the sulfhydryl group and the epsilon-amino group, but reacted preferentially with the sulfhydryl group. As the pH increased from 7.5 to 9.5, the reaction took place mainly between the amino groups and EGDE. By controlling the pH and other conditions, intra- and inter-molecularly cross-linked products with narrow size distribution were obtained. The intramolecular product was about 90% of the reaction mixture. The intermolecularly cross-linked Hb consisted of 74.7% of dimeric Hb with weight-average molecular weights (Mw) of 146 kDa, 18.9% of trimeric Hb with Mw of 228 kDa, and 6.4% of oligomer with four to six Hb units, Mw of 375 kDa. The cross-linking altered the oxygen affinity (P-50) and the Hill coefficient of Hb but they are within the range of hemoglobin-based red cell substitutes. The pI of intramolecularly and intermolecularly cross-linked Hb increased from 6.7-7.4 to 6.9-7.6 and 8.2-8.8 respectively. The study demonstrated that EGDE could be an alternative to glutaraldehyde for cross-linking of hemoglobin and other proteins on a large-scale without significantly impairing the biological activity. (c) 2006 Society of Chemical Industry. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Chemistry, Multidisciplinary ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Chemistry ; Engineering |
| 关键词[WOS] | BLOOD SUBSTITUTES ; POLYMERIZATION ; CHROMATOGRAPHY ; AFFINITY |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000237364500007 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3972]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 2.Beijing Univ Chem Technol, Dept Bioengn, Beijing 100029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, ML,Xu, YH,Zheng, CY,et al. Ethylene gylcol diglycidyl ether as a protein cross-linker: a case study for cross-linking of hemoglobin[J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,2006,81(5):767-775. |
| APA | Lu, ML,Xu, YH,Zheng, CY,Zhangl, GF,&Su, Z.(2006).Ethylene gylcol diglycidyl ether as a protein cross-linker: a case study for cross-linking of hemoglobin.JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,81(5),767-775. |
| MLA | Lu, ML,et al."Ethylene gylcol diglycidyl ether as a protein cross-linker: a case study for cross-linking of hemoglobin".JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 81.5(2006):767-775. |
入库方式： OAI收割
来源：过程工程研究所
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