Expression of a wheat cytochrome P450 monooxygenase cDNA in yeast catalyzes the metabolism of sulfonylurea herbicides
文献类型：期刊论文
| 作者 | Xiang, WS; Wang, XJ; Ren, TR |
| 刊名 | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
 |
| 出版日期 | 2006-05-01 |
| 卷号 | 85期号:1页码:1-6 |
| 关键词 | wheat cytochrome P450 heterologous expression herbicide metabolism sulfonylurea herbicides |
| ISSN号 | 0048-3575 |
| 其他题名 | Pest. Biochem. Physiol. |
| 中文摘要 | A wheat cytochrome P450 cDNA (CYP71C6v1) was cloned by RT-PCR and heterologously expressed in yeast. The microsomal fractions derived from this strain could catalyze the metabolism of some sulfonylurea herbicides such as chlorsulfuron, triasulfuron, metsulfuron-metyl, bensulfuron-metyl, and tribenuron-rnetyl, but not sulfonylurea herbicides such as thifensulfuron and pyrazosulfuron. Kinetic parameters K-m for chlorsulfuron and triasulfuron were 57 (+/- 15)mu M and 38 (+/- 16) mu M in vitro, respectively. Analysis of the metabolites demonstrated that the CYP71C6v1 functioned as a 5-phenyl ring hydroxylase when chlorsulfuron and triasulfuron were the substrates. (c) 2005 Elsevier Inc. All rights reserved. |
| 英文摘要 | A wheat cytochrome P450 cDNA (CYP71C6v1) was cloned by RT-PCR and heterologously expressed in yeast. The microsomal fractions derived from this strain could catalyze the metabolism of some sulfonylurea herbicides such as chlorsulfuron, triasulfuron, metsulfuron-metyl, bensulfuron-metyl, and tribenuron-rnetyl, but not sulfonylurea herbicides such as thifensulfuron and pyrazosulfuron. Kinetic parameters K-m for chlorsulfuron and triasulfuron were 57 (+/- 15)mu M and 38 (+/- 16) mu M in vitro, respectively. Analysis of the metabolites demonstrated that the CYP71C6v1 functioned as a 5-phenyl ring hydroxylase when chlorsulfuron and triasulfuron were the substrates. (c) 2005 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Entomology ; Physiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Entomology ; Physiology |
| 关键词[WOS] | PLANT CYTOCHROME-P450 ; NAPHTHALIC ANHYDRIDE ; MOLECULAR-CLONING ; MAIZE ; TRIASULFURON ; CYP76B1 ; CHLORSULFURON ; HYDROXYLATION ; SELECTIVITY ; MICROSOMES |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000237362900001 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3978]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.NE Agr Univ, Res Ctr Life Sci & Biotechnol, Harbin 150030, Peoples R China 2.Chinese Acad Sci, State Key Lab Biochem Engineer, Inst Proc Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiang, WS,Wang, XJ,Ren, TR. Expression of a wheat cytochrome P450 monooxygenase cDNA in yeast catalyzes the metabolism of sulfonylurea herbicides[J]. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,2006,85(1):1-6. |
| APA | Xiang, WS,Wang, XJ,&Ren, TR.(2006).Expression of a wheat cytochrome P450 monooxygenase cDNA in yeast catalyzes the metabolism of sulfonylurea herbicides.PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,85(1),1-6. |
| MLA | Xiang, WS,et al."Expression of a wheat cytochrome P450 monooxygenase cDNA in yeast catalyzes the metabolism of sulfonylurea herbicides".PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 85.1(2006):1-6. |
入库方式： OAI收割
来源：过程工程研究所
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