Fingerprinting of Salvia miltiorrhiza Bunge by thin-layer chromatography scan compared with high speed countercurrent chromatography
文献类型：期刊论文
| 作者 | Gu, M; Su, ZG; Fan, OY |
| 刊名 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| 出版日期 | 2006 |
| 卷号 | 29期号:10页码:1503-1514 |
| 关键词 | thin-layer chromatography scan ( TLCS) high speed countercurrent chromatography ( HSCCC) fingerprint Salvia miltiorrhiza Bunge tanshinones traditional Chinese medicine ( TCM) |
| ISSN号 | 1082-6076 |
| 其他题名 | J. Liq. Chromatogr. Relat. Technol. |
| 中文摘要 | The components of the traditional Chinese medicines (TCM) can be influenced by soil, climate, and growth stage. Fingerprinting is an important means for its quality control. Our previous studies showed that high speed countercurrent chromatography (HSCCC) was helpful in the development of TCM fingerprinting. Since the HSCCC method is new, it is necessary to compare it with more conventional ones, such as high performance liquid chromatography (HPLC), high performance capillary electrophoresis (HPCE), and thin-layer chromatography scan (TLCS). Comparisons with HPLC and HPCE were reported in our previous studies. In this study, HSCCC was compared with the thin-layer chromatography scan (TLCS). With TLC, a quick, convenient, and cost effective technique, 8 stable components were separated in common within 48 min, respectively, from 3 crude samples of Salvia miltiorrhiza Bunge from different growth locations. In HSCCC separation, 12 components were separated, respectively, with good correspondence and precision within 13 h. Both TLCS and HSCCC were effective in showing the whole concentration distribution of all kinds of constituents in different samples. HSCCC showed better performance in analysis of tanshinones, which produced a fingerprint which contained more chemical information than that of TLCS. It was further proven that HSCCC could be a feasible and cost effective method in the development of the fingerprint of TCM. |
| 英文摘要 | The components of the traditional Chinese medicines (TCM) can be influenced by soil, climate, and growth stage. Fingerprinting is an important means for its quality control. Our previous studies showed that high speed countercurrent chromatography (HSCCC) was helpful in the development of TCM fingerprinting. Since the HSCCC method is new, it is necessary to compare it with more conventional ones, such as high performance liquid chromatography (HPLC), high performance capillary electrophoresis (HPCE), and thin-layer chromatography scan (TLCS). Comparisons with HPLC and HPCE were reported in our previous studies. In this study, HSCCC was compared with the thin-layer chromatography scan (TLCS). With TLC, a quick, convenient, and cost effective technique, 8 stable components were separated in common within 48 min, respectively, from 3 crude samples of Salvia miltiorrhiza Bunge from different growth locations. In HSCCC separation, 12 components were separated, respectively, with good correspondence and precision within 13 h. Both TLCS and HSCCC were effective in showing the whole concentration distribution of all kinds of constituents in different samples. HSCCC showed better performance in analysis of tanshinones, which produced a fingerprint which contained more chemical information than that of TLCS. It was further proven that HSCCC could be a feasible and cost effective method in the development of the fingerprint of TCM. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | PREPARATIVE ISOLATION ; SEPARATION METHODS ; DITERPENOIDS ; PURIFICATION ; TANSHINONES |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000237513000010 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3983]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Process Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gu, M,Su, ZG,Fan, OY. Fingerprinting of Salvia miltiorrhiza Bunge by thin-layer chromatography scan compared with high speed countercurrent chromatography[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2006,29(10):1503-1514. |
| APA | Gu, M,Su, ZG,&Fan, OY.(2006).Fingerprinting of Salvia miltiorrhiza Bunge by thin-layer chromatography scan compared with high speed countercurrent chromatography.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,29(10),1503-1514. |
| MLA | Gu, M,et al."Fingerprinting of Salvia miltiorrhiza Bunge by thin-layer chromatography scan compared with high speed countercurrent chromatography".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 29.10(2006):1503-1514. |
入库方式： OAI收割
来源：过程工程研究所
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