Isolation of lipids from photosystem I complex and its characterization with high performance liquid chromatography/electrospray ionization mass spectrometry
文献类型：期刊论文
| 作者 | Yao, Hongjun; Shi, Yujie; Gao, Rongfu; Zhang, Guifeng; Zhang, Rumin; Zheng, Caixia; Xu, Bingjiu |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2006-06-06 |
| 卷号 | 837期号:1-2页码:101-107 |
| 关键词 | photosystem I lipids reversed-phase high performance liquid chromatography electrospray ionization mass spectrometry |
| ISSN号 | 1570-0232 |
| 其他题名 | J. Chromatogr. B |
| 中文摘要 | A method for simultaneous analysis of lipids extracted from photosystem I complex was developed with high performance liquid chromatography/electrospray ionization mass spectrometry. The photosystem I complex was firstly solubilized and separated using deoxycholate polyacrylamide gel electrophoresis method after ultrasonic treatment of the sample (leaves of pea, Pisum sativum L.). The Photosystem I complexes were electrophoretically eluted from the deoxycholate polyacrylamide gel electrophoresis bands containing them, and the electron transport activity of the eluent measured as confirmation. Lipids, which were isolated from the complex having photosystem I activity, were separated and characterized with high performance liquid chromatography/electrospray ionization mass spectrometry. Five lipids, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, phosphatidylglycerol, sulphoquinovosyldiacylglycerol and phosphaditylcholine were found combining with photosystem I complex. Different species of these lipids were found in the ESI mass spectra and the compositions of the acyl groups in them were determined. (c) 2006 Elsevier B.V. All rights reserved. |
| 英文摘要 | A method for simultaneous analysis of lipids extracted from photosystem I complex was developed with high performance liquid chromatography/electrospray ionization mass spectrometry. The photosystem I complex was firstly solubilized and separated using deoxycholate polyacrylamide gel electrophoresis method after ultrasonic treatment of the sample (leaves of pea, Pisum sativum L.). The Photosystem I complexes were electrophoretically eluted from the deoxycholate polyacrylamide gel electrophoresis bands containing them, and the electron transport activity of the eluent measured as confirmation. Lipids, which were isolated from the complex having photosystem I activity, were separated and characterized with high performance liquid chromatography/electrospray ionization mass spectrometry. Five lipids, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, phosphatidylglycerol, sulphoquinovosyldiacylglycerol and phosphaditylcholine were found combining with photosystem I complex. Different species of these lipids were found in the ESI mass spectra and the compositions of the acyl groups in them were determined. (c) 2006 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | QUANTITATIVE-ANALYSIS ; SEPARATION ; PHOSPHATIDYLCHOLINE ; PHOSPHOLIPIDS ; EXTRACTS ; SYSTEM ; FIELD ; MS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000238311600014 |
| 公开日期 | 2013-10-24 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/4001]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Beijing Forestry Univ, Key Lab Genet & Breeding Forest Trees & Ornamenta, Minist Educ, Beijing 100083, Peoples R China 2.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Beijing 100080, Peoples R China 4.Capital Univ Med Sci, Sch Chem Biol & Pharm Sci, Dept Anal Chem, Beijing 100054, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao, Hongjun,Shi, Yujie,Gao, Rongfu,et al. Isolation of lipids from photosystem I complex and its characterization with high performance liquid chromatography/electrospray ionization mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2006,837(1-2):101-107. |
| APA | Yao, Hongjun.,Shi, Yujie.,Gao, Rongfu.,Zhang, Guifeng.,Zhang, Rumin.,...&Xu, Bingjiu.(2006).Isolation of lipids from photosystem I complex and its characterization with high performance liquid chromatography/electrospray ionization mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,837(1-2),101-107. |
| MLA | Yao, Hongjun,et al."Isolation of lipids from photosystem I complex and its characterization with high performance liquid chromatography/electrospray ionization mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 837.1-2(2006):101-107. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。