中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Isolation of lipids from photosystem I complex and its characterization with high performance liquid chromatography/electrospray ionization mass spectrometry

文献类型:期刊论文

作者Yao, Hongjun; Shi, Yujie; Gao, Rongfu; Zhang, Guifeng; Zhang, Rumin; Zheng, Caixia; Xu, Bingjiu
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
出版日期2006-06-06
卷号837期号:1-2页码:101-107
关键词photosystem I lipids reversed-phase high performance liquid chromatography electrospray ionization mass spectrometry
ISSN号1570-0232
其他题名J. Chromatogr. B
中文摘要A method for simultaneous analysis of lipids extracted from photosystem I complex was developed with high performance liquid chromatography/electrospray ionization mass spectrometry. The photosystem I complex was firstly solubilized and separated using deoxycholate polyacrylamide gel electrophoresis method after ultrasonic treatment of the sample (leaves of pea, Pisum sativum L.). The Photosystem I complexes were electrophoretically eluted from the deoxycholate polyacrylamide gel electrophoresis bands containing them, and the electron transport activity of the eluent measured as confirmation. Lipids, which were isolated from the complex having photosystem I activity, were separated and characterized with high performance liquid chromatography/electrospray ionization mass spectrometry. Five lipids, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, phosphatidylglycerol, sulphoquinovosyldiacylglycerol and phosphaditylcholine were found combining with photosystem I complex. Different species of these lipids were found in the ESI mass spectra and the compositions of the acyl groups in them were determined. (c) 2006 Elsevier B.V. All rights reserved.
英文摘要A method for simultaneous analysis of lipids extracted from photosystem I complex was developed with high performance liquid chromatography/electrospray ionization mass spectrometry. The photosystem I complex was firstly solubilized and separated using deoxycholate polyacrylamide gel electrophoresis method after ultrasonic treatment of the sample (leaves of pea, Pisum sativum L.). The Photosystem I complexes were electrophoretically eluted from the deoxycholate polyacrylamide gel electrophoresis bands containing them, and the electron transport activity of the eluent measured as confirmation. Lipids, which were isolated from the complex having photosystem I activity, were separated and characterized with high performance liquid chromatography/electrospray ionization mass spectrometry. Five lipids, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, phosphatidylglycerol, sulphoquinovosyldiacylglycerol and phosphaditylcholine were found combining with photosystem I complex. Different species of these lipids were found in the ESI mass spectra and the compositions of the acyl groups in them were determined. (c) 2006 Elsevier B.V. All rights reserved.
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]QUANTITATIVE-ANALYSIS ; SEPARATION ; PHOSPHATIDYLCHOLINE ; PHOSPHOLIPIDS ; EXTRACTS ; SYSTEM ; FIELD ; MS
收录类别SCI
原文出处://WOS:000238311600014
语种英语
WOS记录号WOS:000238311600014
公开日期2013-10-24
版本出版稿
源URL[http://ir.ipe.ac.cn/handle/122111/4001]  
专题过程工程研究所_研究所(批量导入)
作者单位1.Beijing Forestry Univ, Key Lab Genet & Breeding Forest Trees & Ornamenta, Minist Educ, Beijing 100083, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Beijing 100080, Peoples R China
4.Capital Univ Med Sci, Sch Chem Biol & Pharm Sci, Dept Anal Chem, Beijing 100054, Peoples R China
推荐引用方式
GB/T 7714
Yao, Hongjun,Shi, Yujie,Gao, Rongfu,et al. Isolation of lipids from photosystem I complex and its characterization with high performance liquid chromatography/electrospray ionization mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2006,837(1-2):101-107.
APA Yao, Hongjun.,Shi, Yujie.,Gao, Rongfu.,Zhang, Guifeng.,Zhang, Rumin.,...&Xu, Bingjiu.(2006).Isolation of lipids from photosystem I complex and its characterization with high performance liquid chromatography/electrospray ionization mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,837(1-2),101-107.
MLA Yao, Hongjun,et al."Isolation of lipids from photosystem I complex and its characterization with high performance liquid chromatography/electrospray ionization mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 837.1-2(2006):101-107.

入库方式: OAI收割

来源:过程工程研究所

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